Genomic comparison of mecC-carrying methicillin-resistant Staphylococcus aureus from hedgehogs and humans in the Netherlands

Dutch MRSA surveillance study group; Cindy Dierikx; Paul Hengeveld; Sandra Witteveen; Angela van Hoek; Marga van Santen-Verheuvel; Margriet Montizaan; Marja Kik; Miriam Maas; Leo Schouls; Antoni Hendrickx; Engeline van Duijkeren

doi:10.1093/jac/dkad047

Genomic comparison of mecC-carrying methicillin-resistant Staphylococcus aureus from hedgehogs and humans in the Netherlands

Dutch MRSA surveillance study group, Cindy Dierikx^*, Paul Hengeveld, Sandra Witteveen, Angela van Hoek, Marga van Santen-Verheuvel, Margriet Montizaan, Marja Kik, Miriam Maas, Leo Schouls, Antoni Hendrickx, Engeline van Duijkeren

^*Corresponding author voor dit werk

Onderzoeksoutput › Academic › peer review

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OBJECTIVES: MRSA carrying the mecC gene (mecC-MRSA) have been found in humans and animals worldwide. A high carriage rate of mecC-MRSA has been described among hedgehogs in different countries. We performed genomic comparison of mecC-MRSA from hedgehogs and humans using next-generation sequencing (NGS) to investigate possible zoonotic transmission in the Netherlands.

METHODS: Nasal swabs from hedgehogs (n = 105) were cultured using pre-enrichment and selective plates. Isolates were sequenced using Illumina NGS platforms. These data were compared with sequence data of mecC-MRSA (n = 62) from the Dutch national MRSA surveillance in humans.

RESULTS: Fifty hedgehogs were found to be MRSA positive, of which 48 carried mecC. A total of 60 mecC-MRSA isolates derived from 50 hedgehogs were compared with the human isolates. Fifty-nine mecC-MRSA from hedgehogs and all but one isolate from humans belonged to clonal complexes CC130 and CC1943. The mecC gene was located within the SCCmec XI element. Most mecC-MRSA did not carry other resistance genes besides mecC and blaZ. Two human isolates carried erm(C). Isolates differed in the presence of various virulence genes, which were linked to distinct STs and clonal complexes. Some isolates had up to 17 virulence genes, which underlines their pathogenic potential. No genetic clusters of hedgehog and human isolates were found.

CONCLUSIONS: mecC-MRSA from hedgehogs and humans mainly belonged to the same two clonal complexes, indicating a common source. No firm evidence for recent zoonotic transmission was found. Further studies are needed to investigate the role of hedgehogs in the occurrence of mecC-MRSA in humans.

Originele taal-2	English
Pagina's (van-tot)	1168-1174
Aantal pagina's	7
Tijdschrift	The Journal of antimicrobial chemotherapy
Volume	78
Nummer van het tijdschrift	5
DOI's	https://doi.org/10.1093/jac/dkad047
Status	Published - 3-mei-2023

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10.1093/jac/dkad047

Genomic comparison of mecC-carrying methicillin-resistant Staphylococcus aureus from hedgehogs and humans in the NetherlandsFinal publisher's version, 432 KBLicentie: Taverne

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Dutch MRSA surveillance study group, Dierikx, C., Hengeveld, P., Witteveen, S., van Hoek, A., van Santen-Verheuvel, M., Montizaan, M., Kik, M., Maas, M., Schouls, L., Hendrickx, A., & van Duijkeren, E. (2023). Genomic comparison of mecC-carrying methicillin-resistant Staphylococcus aureus from hedgehogs and humans in the Netherlands. The Journal of antimicrobial chemotherapy, 78(5), 1168-1174. https://doi.org/10.1093/jac/dkad047

@article{be5ee8b695944ac8bcdb336f41b65b3e,

title = "Genomic comparison of mecC-carrying methicillin-resistant Staphylococcus aureus from hedgehogs and humans in the Netherlands",

abstract = "OBJECTIVES: MRSA carrying the mecC gene (mecC-MRSA) have been found in humans and animals worldwide. A high carriage rate of mecC-MRSA has been described among hedgehogs in different countries. We performed genomic comparison of mecC-MRSA from hedgehogs and humans using next-generation sequencing (NGS) to investigate possible zoonotic transmission in the Netherlands.METHODS: Nasal swabs from hedgehogs (n = 105) were cultured using pre-enrichment and selective plates. Isolates were sequenced using Illumina NGS platforms. These data were compared with sequence data of mecC-MRSA (n = 62) from the Dutch national MRSA surveillance in humans.RESULTS: Fifty hedgehogs were found to be MRSA positive, of which 48 carried mecC. A total of 60 mecC-MRSA isolates derived from 50 hedgehogs were compared with the human isolates. Fifty-nine mecC-MRSA from hedgehogs and all but one isolate from humans belonged to clonal complexes CC130 and CC1943. The mecC gene was located within the SCCmec XI element. Most mecC-MRSA did not carry other resistance genes besides mecC and blaZ. Two human isolates carried erm(C). Isolates differed in the presence of various virulence genes, which were linked to distinct STs and clonal complexes. Some isolates had up to 17 virulence genes, which underlines their pathogenic potential. No genetic clusters of hedgehog and human isolates were found.CONCLUSIONS: mecC-MRSA from hedgehogs and humans mainly belonged to the same two clonal complexes, indicating a common source. No firm evidence for recent zoonotic transmission was found. Further studies are needed to investigate the role of hedgehogs in the occurrence of mecC-MRSA in humans.",

keywords = "Animals, Humans, Methicillin-Resistant Staphylococcus aureus/genetics, Netherlands/epidemiology, Bacterial Proteins/genetics, Staphylococcal Infections/epidemiology, Hedgehog Proteins, Genomics, Microbial Sensitivity Tests",

author = "{Dutch MRSA surveillance study group} and Cindy Dierikx and Paul Hengeveld and Sandra Witteveen and {van Hoek}, Angela and {van Santen-Verheuvel}, Marga and Margriet Montizaan and Marja Kik and Miriam Maas and Leo Schouls and Antoni Hendrickx and {van Duijkeren}, Engeline and Erik Bathoorn",

note = "{\textcopyright} The Author(s) 2023. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, please e-mail: [email protected].",

year = "2023",

month = may,

day = "3",

doi = "10.1093/jac/dkad047",

language = "English",

volume = "78",

pages = "1168--1174",

journal = "The Journal of antimicrobial chemotherapy",

issn = "0305-7453",

publisher = "Oxford University Press",

number = "5",

}

Dutch MRSA surveillance study group, Dierikx, C, Hengeveld, P, Witteveen, S, van Hoek, A, van Santen-Verheuvel, M, Montizaan, M, Kik, M, Maas, M, Schouls, L, Hendrickx, A & van Duijkeren, E 2023, 'Genomic comparison of mecC-carrying methicillin-resistant Staphylococcus aureus from hedgehogs and humans in the Netherlands', The Journal of antimicrobial chemotherapy, vol. 78, nr. 5, blz. 1168-1174. https://doi.org/10.1093/jac/dkad047

TY - JOUR

T1 - Genomic comparison of mecC-carrying methicillin-resistant Staphylococcus aureus from hedgehogs and humans in the Netherlands

AU - Dutch MRSA surveillance study group

AU - Dierikx, Cindy

AU - Hengeveld, Paul

AU - Witteveen, Sandra

AU - van Hoek, Angela

AU - van Santen-Verheuvel, Marga

AU - Montizaan, Margriet

AU - Kik, Marja

AU - Maas, Miriam

AU - Schouls, Leo

AU - Hendrickx, Antoni

AU - van Duijkeren, Engeline

AU - Bathoorn, Erik

PY - 2023/5/3

Y1 - 2023/5/3

N2 - OBJECTIVES: MRSA carrying the mecC gene (mecC-MRSA) have been found in humans and animals worldwide. A high carriage rate of mecC-MRSA has been described among hedgehogs in different countries. We performed genomic comparison of mecC-MRSA from hedgehogs and humans using next-generation sequencing (NGS) to investigate possible zoonotic transmission in the Netherlands.METHODS: Nasal swabs from hedgehogs (n = 105) were cultured using pre-enrichment and selective plates. Isolates were sequenced using Illumina NGS platforms. These data were compared with sequence data of mecC-MRSA (n = 62) from the Dutch national MRSA surveillance in humans.RESULTS: Fifty hedgehogs were found to be MRSA positive, of which 48 carried mecC. A total of 60 mecC-MRSA isolates derived from 50 hedgehogs were compared with the human isolates. Fifty-nine mecC-MRSA from hedgehogs and all but one isolate from humans belonged to clonal complexes CC130 and CC1943. The mecC gene was located within the SCCmec XI element. Most mecC-MRSA did not carry other resistance genes besides mecC and blaZ. Two human isolates carried erm(C). Isolates differed in the presence of various virulence genes, which were linked to distinct STs and clonal complexes. Some isolates had up to 17 virulence genes, which underlines their pathogenic potential. No genetic clusters of hedgehog and human isolates were found.CONCLUSIONS: mecC-MRSA from hedgehogs and humans mainly belonged to the same two clonal complexes, indicating a common source. No firm evidence for recent zoonotic transmission was found. Further studies are needed to investigate the role of hedgehogs in the occurrence of mecC-MRSA in humans.

AB - OBJECTIVES: MRSA carrying the mecC gene (mecC-MRSA) have been found in humans and animals worldwide. A high carriage rate of mecC-MRSA has been described among hedgehogs in different countries. We performed genomic comparison of mecC-MRSA from hedgehogs and humans using next-generation sequencing (NGS) to investigate possible zoonotic transmission in the Netherlands.METHODS: Nasal swabs from hedgehogs (n = 105) were cultured using pre-enrichment and selective plates. Isolates were sequenced using Illumina NGS platforms. These data were compared with sequence data of mecC-MRSA (n = 62) from the Dutch national MRSA surveillance in humans.RESULTS: Fifty hedgehogs were found to be MRSA positive, of which 48 carried mecC. A total of 60 mecC-MRSA isolates derived from 50 hedgehogs were compared with the human isolates. Fifty-nine mecC-MRSA from hedgehogs and all but one isolate from humans belonged to clonal complexes CC130 and CC1943. The mecC gene was located within the SCCmec XI element. Most mecC-MRSA did not carry other resistance genes besides mecC and blaZ. Two human isolates carried erm(C). Isolates differed in the presence of various virulence genes, which were linked to distinct STs and clonal complexes. Some isolates had up to 17 virulence genes, which underlines their pathogenic potential. No genetic clusters of hedgehog and human isolates were found.CONCLUSIONS: mecC-MRSA from hedgehogs and humans mainly belonged to the same two clonal complexes, indicating a common source. No firm evidence for recent zoonotic transmission was found. Further studies are needed to investigate the role of hedgehogs in the occurrence of mecC-MRSA in humans.

KW - Animals

KW - Humans

KW - Methicillin-Resistant Staphylococcus aureus/genetics

KW - Netherlands/epidemiology

KW - Bacterial Proteins/genetics

KW - Staphylococcal Infections/epidemiology

KW - Hedgehog Proteins

KW - Genomics

KW - Microbial Sensitivity Tests

U2 - 10.1093/jac/dkad047

DO - 10.1093/jac/dkad047

M3 - Article

C2 - 36995977

SN - 0305-7453

VL - 78

SP - 1168

EP - 1174

JO - The Journal of antimicrobial chemotherapy

JF - The Journal of antimicrobial chemotherapy

IS - 5

ER -

Genomic comparison of mecC-carrying methicillin-resistant Staphylococcus aureus from hedgehogs and humans in the Netherlands

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