Germinal centres and the B-cell system. V: Presence of germinal centre-precursor cells among lymphocytes of the thoracic duct in the rat

Nicolette A. Gastkemper*, Auk S. Wubbena, Felix J. H. Gimbrère, Alexander de Graaff, Paul Nieuwenhuis

*Bijbehorende auteur voor dit werk

    OnderzoeksoutputAcademicpeer review

    22 Citaten (Scopus)

    Samenvatting

    Thoracic duct lymphocytes (TDL) were studied with respect to their capacity to give rise to germinal centres (GC) and to form primary antibody in an adoptive transfer system of the rat. Challenge with sheep erythrocytes (SRBC) 24h after lethal irradiation (900 rads) and syngeneic TDL reconstitution (108) lead to conspicuous GC activity already 7 days after transfer. In contrast, using syngeneic bone marrow (BM) in the adoptive transfer system, no GC formation was observed over the period studied (14 days after reconstitution). Reconstitution experiments using in vivo-separated T-TDL (1–5 % s-Ig+) and B-TDL (>90% s-Ig+) subpopulations, either separately or in combination, indicated that GC originate from B-TDL but require T-TDL for induction.
    Originele taal-2English
    Pagina's (van-tot)281-289
    Aantal pagina's9
    TijdschriftCell and Tissue Research
    Volume219
    Nummer van het tijdschrift2
    DOI's
    StatusPublished - 1981

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