Hematopoietic stem cell expansion: challenges and opportunities

Marta A. Walasek, Ronald van Os, Gerald de Haan*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

143 Citaten (Scopus)

Samenvatting

Attempts to improve hematopoietic reconstitution and engraftment potential of ex vivo-expanded hematopoietic stem and progenitor cells (HSPCs) have been largely unsuccessful due to the inability to generate sufficient stem cell numbers and to excessive differentiation of the starting cell population. Although hematopoietic stem cells (HSCs) will rapidly expand after in vivo transplantation, experience from in vitro studies indicates that control of HSPC self-renewal and differentiation in culture remains difficult. Protocols that are based on hematopoietic cytokines have failed to support reliable amplification of immature stem cells in culture, suggesting that additional factors are required. In recent years, several novel factors, including developmental factors and chemical compounds, have been reported to affect HSC self-renewal and improve ex vivo stem cell expansion protocols. Here, we highlight early expansion attempts and review recent development in the extrinsic control of HSPC fate in vitro.

Originele taal-2English
TitelHEMATOPOIETIC STEM CELLS VIII
RedacteurenL Kanz, WE Fibbe, C Lengerke, JE Dick
Plaats van productieOXFORD
UitgeverijBlackwell Publishing
Pagina's138-150
Aantal pagina's13
ISBN van geprinte versie978-1-57331-867-9
DOI's
StatusPublished - 2012
Evenement8th International Conference and Workshop on Hematopoietic Stem Cells - , Germany
Duur: 22-sep-201124-sep-2011

Publicatie series

NaamAnnals of the New York Academy of Sciences
UitgeverijBLACKWELL SCIENCE PUBL
Volume1266
ISSN van geprinte versie0077-8923

Other

Other8th International Conference and Workshop on Hematopoietic Stem Cells
Land/RegioGermany
Periode22/09/201124/09/2011

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