High Loading Efficiency and Controlled Release of Bioactive Immunotherapeutic Proteins Using Vaterite Nanoparticles

Ghizlane Choukrani, Jimena Alvarez Freile, Natasha Ustyanovska Avtenyuk, Wei Wan, Kerstin Zimmermann, Edwin Bremer, Lars Dahne*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

3 Downloads (Pure)

Samenvatting

Nanoparticles may limit off-tumor/on-target ubiquitous activation of signaling by protein-based drugs. However, many challenges still exist in the design of a nanoparticle for protein delivery. In this study, conditions to establish vaterite nanoparticles as a pH-sensitive drug delivery system (DDS) for encapsulated protein drugs are comprehensively evaluated. Low coprecipitation pH of vaterite and protein prevents protein denaturation and yields high loading efficiency. Unprotected vaterite recrystallizes in aqueous solutions within 3 h to calcite and releases the loaded protein completely, but surface-modified particles with carboxyl groups containing polymers prove stable for more than 5 months. Notably, modification of vaterite with sulfonated polymers increases the loading of cationic proteins by a multiple. A system is developed for vaterite exposure to (pH) conditions under body-like-flow rates, with the dissolution of vaterite and simultaneous release of active proteins at tumor microenvironmental pH reaching up to 80% and only 20% at physiological pH within 2 h. Importantly, the immunomodulatory protein tumor necrosis factor preserves its native structure and fully retains functional activity in vitro after release from the particles. In conclusion, the studies described here provide a framework for the development of vaterite-based DDS as a carrier for bioactive protein-based therapeutics.

Originele taal-2English
Artikelnummer2100012
Pagina's (van-tot)1-10
Aantal pagina's10
TijdschriftParticle & Particle Systems Characterization
DOI's
StatusE-pub ahead of print - 12-jun-2021

Citeer dit