Hole Migration In Telomere-Based Oligonucleotide Anions And G-Quadruplexes

Wen Li, Edita Mjekiqi, Wessel Douma, Xin Wang, Oksana Kavatsyuk, Ronnie Hoekstra, Jean-Christophe Poully, Thomas Schlathölter*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

7 Citaten (Scopus)
115 Downloads (Pure)


Vacuum ultraviolet photoionization of a gas-phase oligonucleotide anion leads to the formation of a valence hole. This hole migrates towards an energetically favorable site where it can weaken bonds and ultimately lead to bond cleavage. We have studied VUV photoionization of deprotonated oligonucleotides containing the human telomere sequence dTTAGGG and G-quadruplex structures consisting of four dTGGGGT single strands, stabilized by NH4+ counter ions. The oligonucleotide and G-quadruplex anions were confined in a radiofrequency ion trap, interfaced with a synchrotron beamline and the photofragmentation was studied using time-of-flight mass spectrometry. Oligonucleotide 12-mers containing the 5´-TTAGGG sequence were found to predominantly break in the GGG region, whereas no selective bond cleavage region was observed for the reversed 5´-GGGATT sequence. For G-quadruplex structures, fragmentation was quenched and mostly non-dissociative single and double electron removal was observed.
Originele taal-2English
Pagina's (van-tot)16114 –16119
Aantal pagina's6
TijdschriftChemistry - A European Journal
Nummer van het tijdschrift70
StatusPublished - 15-okt.-2019

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