Identification of seipin-linked factors that act as determinants of a lipid droplet subpopulation

Michal Eisenberg-Bord, Muriel Mari, Uri Weill, Eden Rosenfeld-Gur, Ofer Moldavski, Ines G. Castro, Krishnakant G. Soni, Nofar Harpaz, Tim P. Levine, Anthony H. Futerman, Fulvio Reggiori, Vytas A. Bankaitis, Maya Schuldiner, Maria Bohnert

OnderzoeksoutputAcademicpeer review

53 Citaten (Scopus)
304 Downloads (Pure)

Samenvatting

Functional heterogeneity within the lipid droplet (LD) pool of a single cell has been observed, yet the underlying mechanisms remain enigmatic. Here, we report on identification of a specialized LD subpopulation characterized by a unique proteome and a defined geographical location at the nucleus-vacuole junction contact site. In search for factors determining identity of these LDs, we screened similar to 6,000 yeast mutants for loss of targeting of the subpopulation marker Pdr16 and identified Ldo45 (LD organization protein of 45 kD) as a crucial targeting determinant. Ldo45 is the product of a splicing event connecting two adjacent genes (YMR147W and YMR148W/OSW5/LDO16). We show that Ldo proteins cooperate with the LD biogenesis component seipin and establish LD identity by defining positioning and surface-protein composition. Our studies suggest a mechanism to establish functional differentiation of organelles, opening the door to better understanding of metabolic decisions in cells.

Originele taal-2English
Pagina's (van-tot)269-282
Aantal pagina's14
TijdschriftThe Journal of Cell Biology
Volume217
Nummer van het tijdschrift1
Vroegere onlinedatum29-nov-2017
DOI's
StatusPublished - jan-2018

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