IFDOTMETER: A New Software Application for Automated Immunofluorescence Analysis

Mario Rodriguez-Arribas, Elisa Pizarro-Estrella, Rubén Gómez-Sánchez, S. M. S. Yakhine-Diop, Antonio Gragera-Hidalgo, Alejandro Cristo, Jose M. Bravo-San Pedro, Rosa A. Gonzalez-Polo, Jose M. Fuentes*

*Corresponding author voor dit werk

OnderzoeksoutputAcademicpeer review

9 Citaten (Scopus)

Samenvatting

Most laboratories interested in autophagy use different imaging software for managing and analyzing heterogeneous parameters in immunofluorescence experiments (e.g., LC3-puncta quantification and determination of the number and size of lysosomes). One solution would be software that works on a user's laptop or workstation that can access all image settings and provide quick and easy-to-use analysis of data. Thus, we have designed and implemented an application called IFDOTMETER, which can run on all major operating systems because it has been programmed using JAVA (Sun Microsystems). Briefly, IFDOTMETER software has been created to quantify a variety of biological hallmarks, including mitochondrial morphology and nuclear condensation. The program interface is intuitive and user-friendly, making it useful for users not familiar with computer handling. By setting previously defined parameters, the software can automatically analyze a large number of images without the supervision of the researcher. Once analysis is complete, the results are stored in a spreadsheet. Using software for high-throughput cell image analysis offers researchers the possibility of performing comprehensive and precise analysis of a high number of images in an automated manner, making this routine task easier.

Originele taal-2English
Pagina's (van-tot)246-259
Aantal pagina's14
TijdschriftJournal of Laboratory Automation
Volume21
Nummer van het tijdschrift2
Vroegere onlinedatum24-aug.-2015
DOI's
StatusPublished - apr.-2016
Extern gepubliceerdJa

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