Impact of ancestral sequence reconstruction on mechanistic and structural enzymology

Callum R Nicoll; Marta Massari; Marco W Fraaije; Maria Laura Mascotti; Andrea Mattevi

doi:10.1016/j.sbi.2023.102669

Impact of ancestral sequence reconstruction on mechanistic and structural enzymology

Callum R Nicoll, Marta Massari, Marco W Fraaije, Maria Laura Mascotti, Andrea Mattevi^*

^*Corresponding author voor dit werk

Biotechnologie

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Ancestral sequence reconstruction (ASR) provides insight into the changes within a protein sequence across evolution. More specifically, it can illustrate how specific amino acid changes give rise to different phenotypes within a protein family. Over the last few decades it has established itself as a powerful technique for revealing molecular common denominators that govern enzyme function. Here, we describe the strength of ASR in unveiling catalytic mechanisms and emerging phenotypes for a range of different proteins, also highlighting biotechnological applications the methodology can provide.

Originele taal-2	English
Artikelnummer	102669
Aantal pagina's	8
Tijdschrift	Current Opinion in Structural Biology
Volume	82
Vroegere onlinedatum	4-aug.-2023
DOI's	https://doi.org/10.1016/j.sbi.2023.102669
Status	Published - okt.-2023

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10.1016/j.sbi.2023.102669Licentie: CC BY-NC-ND

Impact of ancestral sequence reconstruction on mechanistic and structural enzymologyFinal publisher's version, 1,4 MBLicentie: CC BY-NC-ND

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oLife Fellowship Programme
Roos, W. (Hoofdonderzoeker), van der Tak, F. (Hoofdonderzoeker), Zijlstra, W. (Hoofdonderzoeker), Dobos, V. (Postdoc), Heinen, L. (Postdoc), Thangaratnarajah, C. (Postdoc), Hoekzema, M. (Postdoc), Blokhuis, A. (Postdoc), Mascotti, L. (Postdoc), Padin Santos, D. (Postdoc), Chopra, A. (Postdoc), Obermaier, S. (Postdoc), Driver, M. (Postdoc), Moreira Goulart, M. (Postdoc), Sasidharan, S. (Postdoc), Samar Mahapatra, S. (Postdoc), Zylstra, A. (Postdoc), Geiger, Y. (Postdoc), Llopis Lorente, A. (Postdoc), Aschmann, D. (Postdoc) & Kulala Vittala, S. (Postdoc)
01/04/2019 → 31/03/2024
Project: Research

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abstract = "Ancestral sequence reconstruction (ASR) provides insight into the changes within a protein sequence across evolution. More specifically, it can illustrate how specific amino acid changes give rise to different phenotypes within a protein family. Over the last few decades it has established itself as a powerful technique for revealing molecular common denominators that govern enzyme function. Here, we describe the strength of ASR in unveiling catalytic mechanisms and emerging phenotypes for a range of different proteins, also highlighting biotechnological applications the methodology can provide.",

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AU - Nicoll, Callum R

AU - Massari, Marta

AU - Fraaije, Marco W

AU - Mascotti, Maria Laura

AU - Mattevi, Andrea

PY - 2023/10

Y1 - 2023/10

N2 - Ancestral sequence reconstruction (ASR) provides insight into the changes within a protein sequence across evolution. More specifically, it can illustrate how specific amino acid changes give rise to different phenotypes within a protein family. Over the last few decades it has established itself as a powerful technique for revealing molecular common denominators that govern enzyme function. Here, we describe the strength of ASR in unveiling catalytic mechanisms and emerging phenotypes for a range of different proteins, also highlighting biotechnological applications the methodology can provide.

AB - Ancestral sequence reconstruction (ASR) provides insight into the changes within a protein sequence across evolution. More specifically, it can illustrate how specific amino acid changes give rise to different phenotypes within a protein family. Over the last few decades it has established itself as a powerful technique for revealing molecular common denominators that govern enzyme function. Here, we describe the strength of ASR in unveiling catalytic mechanisms and emerging phenotypes for a range of different proteins, also highlighting biotechnological applications the methodology can provide.

U2 - 10.1016/j.sbi.2023.102669

DO - 10.1016/j.sbi.2023.102669

M3 - Review article

C2 - 37544113

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VL - 82

JO - Current Opinion in Structural Biology

JF - Current Opinion in Structural Biology

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Impact of ancestral sequence reconstruction on mechanistic and structural enzymology

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