In vitro generation of long-term repopulating hematopoietic stem cells by fibroblast growth factor-1

G de Haan*, E Weersing, B Dontje, R van Os, LV Bystrykh, E Vellenga, G Miller

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

123 Citaten (Scopus)

Samenvatting

The role of fibroblast growth factors and their receptors (FGFRs) in the regulation of normal hematopoietic stem cells is unknown. Here we show that, in mouse bone marrow, long-term repopulating stem cells are found exclusively in the FGFR(+) cell fraction. During differentiation toward committed progenitors, stem cells show loss of FGFR expression. Prolonged culture of bone marrow cells in serum-free medium supplemented with only FGF-1 resulted in robust expansion of multilineage, serially transplantable, long-term repopulating hematopoietic stem cells. Thus, we have identified a simple method of generating large numbers of rapidly engrafting stem cells that have not been genetically manipulated. Our results show that the multipotential properties of stem cells are dependent on signaling through FGF receptors and that FGF-1 plays an important role in hematopoietic stem cell homeostasis.

Originele taal-2English
Pagina's (van-tot)241-251
Aantal pagina's11
TijdschriftDevelopmental Cell
Volume4
Nummer van het tijdschrift2
StatusPublished - feb-2003

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