Influence of Miscibility of Protein-Sugar Lyophilizates on Their Storage Stability

Maarten A. Mensink, Matthew J. Nethercott, Wouter L. J. Hinrichs, Kees van der Voort Maarschalk, Henderik W. Frijlink, Eric J. Munson, Michael J. Pikal

OnderzoeksoutputAcademicpeer review

22 Citaten (Scopus)
242 Downloads (Pure)


For sugars to act as successful stabilizers of proteins during lyophilization and subsequent storage, they need to have several characteristics. One of them is that they need to be able to form interactions with the protein and for that miscibility is essential. To evaluate the influence of protein-sugar miscibility on protein storage stability, model protein IgG was lyophilized in the presence of various sugars of different molecular weight. By comparing solid-state nuclear magnetic resonance spectroscopy relaxation times of both protein and sugar on two different timescales, i.e., H-1 T-1 and H-1 T-1 rho, miscibility of the two components was established on a 2-5- and a 20-50-nm length scale, respectively, and related to protein storage stability. Smaller sugars showed better miscibility with IgG, and the tendency of IgG to aggregate during storage was lower for smaller sugars. The largest sugar performed worst and was phase separated on both length scales. Additionally, shorter protein H-1 T-1 relaxation times correlated with higher aggregation rates during storage. The enzyme-linked immunosorbent assay (ELISA) assay showed overlapping effects of aggregation and chemical degradation and did not correspond as well with the miscibility. Because of the small scale at which miscibility was determined (2-5 nm) and the size of the protein domains (similar to 2.5 x 2.5 x 5 nm), the miscibility data give an indirect measure of interaction between protein and sugar. This reduced interaction could be the result of steric hindrance, providing a possible explanation as to why smaller sugars show better miscibility and storage stability with the protein.

Originele taal-2English
Pagina's (van-tot)1225-1232
Aantal pagina's8
TijdschriftAaps journal
Nummer van het tijdschrift5
Vroegere onlinedatum14-jun-2016
StatusPublished - sep-2016

Citeer dit