Influence of temperature on the kinetics absorption of carbon dioxide in aqueous of enzyme catalysed MDEA solutions

In the present work the absorption of carbon dioxide in 1000 mol m(-3) N-methyldiethanolamine (MDEA) solutions with and without enzyme has been studied in a stirred cell reactor in the temperature range 278-343 K and enzyme concentrations ranging from 0 to 1600 gm(-3). During this study a new type of carbonic anhydrase has been tested: the evolved tetrameric enzyme GS6-046. The kinetic data from the new enzyme has been compared to the kinetic data obtained with the thermostable CA mutant 5X and the new enzyme showed a faster kinetics towards the CO2 hydration in combination with MDEA.

The experimental kinetic data from the evolved CA at the temperatures 278, 288 and 313 K resulted in a temperature dependent equation for the enzymatic rate constant K-H2O*. In the temperature range 278-313 K, this equation predicts k(H2O)* within an accuracy of 30%. However, at 343 K the measured k(H2O)* is significantly overestimated. (C) 2016 Elsevier Ltd. All rights reserved.