Samenvatting
Background
Interleukin-6 (IL-6) levels are upregulated in myocardial infarction. Recent data suggest a causal role of the IL-6 receptor (IL-6R) in coronary heart disease. We evaluated if IL-6R blockade by a monoclonal antibody (MR16-1) prevents the heart from adverse left ventricular remodeling in a mouse model of ischemia-reperfusion (I/R).
Methods
CJ57/BL6 mice underwent I/R injury (left coronary artery ligation for 45 minutes) or sham surgery, and thereafter received MR16-1 (2mg/mouse) 5 minutes before reperfusion and 0.5mg/mouse weekly during four weeks, or control IgG treatment. Cardiac Magnetic Resonance Imaging (CMR) and hemodynamic measurements were performed to determine cardiac function after four weeks.
Results
I/R caused left ventricular dilatation and a decrease in left ventricular ejection fraction (LVEF). However, LVEF was significantly lower in the MR16-1 treatment group compared to the IgG group (28 +/- 4% vs. 35 +/- 6%, p = 0.02; sham 45 +/- 6% vs. 43 +/- 4%, respectively; p = NS). Cardiac relaxation (assessed by dP/dT) was not significantly different between the MR16-1 and IgG groups. Also, no differences were observed in histological myocardial fibrosis, infarct size and myocyte hypertrophy between the groups.
Conclusion
Blockade of the IL-6R receptor by the monoclonal MR16-1 antibody for four weeks started directly after I/R injury did not prevent the process of cardiac remodeling in mice, but rather associated with a deterioration in the process of adverse cardiac remodeling.
Originele taal-2 | English |
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Artikelnummer | e0167195 |
Aantal pagina's | 11 |
Tijdschrift | PLoS ONE |
Volume | 11 |
Nummer van het tijdschrift | 12 |
DOI's | |
Status | Published - 9-dec.-2016 |
Vingerafdruk
Duik in de onderzoeksthema's van 'Inhibition of Interleukin-6 Receptor in a Murine Model of Myocardial Ischemia-Reperfusion'. Samen vormen ze een unieke vingerafdruk.Datasets
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Datasets: Inhibition of Interleukin-6 Receptor in a Murine Model of Myocardial Ischemia-Reperfusion
Hartman, H. (Creator), Vreeswijk-Baudoin, I. (Creator), Groot, H. (Creator), de Kolk, K. W. V. (Creator), de Boer, R. (Creator), Mateo Leach, I. (Creator), Vliegenthart, R. (Creator), Sillje, H. (Creator) & van der Harst, P. (Creator), University of Groningen, 18-nov.-2016
DOI: 10.6084/m9.figshare.4239137.v1, https://figshare.com/articles/IL6R_experiment_data_final_17nov2016_dta/4239137 en nog één link, https://figshare.com/articles/IL6R_experiment_data_final_17nov2016_dta/4239137/1 (minder tonen)
Dataset