Insights into the transport mechanism of energy-coupling factor transporters

Weronika Karolina Stanek

Insights into the transport mechanism of energy-coupling factor transporters

Weronika Karolina Stanek

Enzymologie

Onderzoeksoutput: Thesis fully internal (DIV)

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Samenvatting

Vitamines zijn essentiële nutriënten voor organismen. Veel organismen zijn afhankelijk van cofactoren die worden opgenomen vanuit de omgeving. Hoewel er bio-synthetische routes voor de productie van deze moleculen zijn hebben sommige organismen geen complete bio-synthetische route ontwikkeld en halen de bouwstenen hierom uit de extracellulaire omgeving. Hiervoor vindt selectieve opname plaats d.m.v. transporteiwitten in het celmembraan. Het belang van transportsystemen voor de opname van nutriënten is zichtbaar in de hoeveelheid genen die transleren voor transporteiwitten in het prokaryotische genoom (tot wel 16%). Transporters zijn eiwitten die vaak geoptimaliseerd zijn voor de opname van specifieke substraten of een groep substraten. De mechanismen onderliggend aan het transport kunnen daarbij sterk variëren. Het is enorm interessant hoe in de natuur verschillende oplossingen zijn geëvolueerd voor het transport van zo’n diversiteit van opgeloste stoffen. Ons werk concentreert zich rondom Energy-Coupling Factor (ECF) transporters. Deze unieke transporters gebruiken de energie opgeslagen in de vorm van ATP om het transport van vitamines en micronutriënten over het lipiden membraan te transporteren.

Originele taal-2	English
Kwalificatie	Doctor of Philosophy
Toekennende instantie	Rijksuniversiteit Groningen
Begeleider(s)/adviseur	Slotboom, Dirk, Supervisor Poolman, Berend, Supervisor
Datum van toekenning	26-okt.-2018
Plaats van publicatie	[Groningen]
Uitgever	University of Groningen
Gedrukte ISBN's	978-94-034-1052-4
Elektronische ISBN's	978-94-034-1051-7
Status	Published - 2018

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Title and contentsFinal publisher's version, 250 KB
Chapter 1Final publisher's version, 1,04 MB
Chapter 2Final publisher's version, 1,04 MB
Chapter 3Final publisher's version, 1,1 MB
Chapter 4Final publisher's version, 1,84 MB
Chapter 5Final publisher's version, 3,8 MB
Chapter 6Final publisher's version, 1,4 MB
Chapter 7Final publisher's version, 1,96 MB
Chapter 8Final publisher's version, 3,87 MB
Supplementary tablesFinal publisher's version, 780 KB
Conclusions and outlookFinal publisher's version, 415 KB
Nederlandse samenvattingFinal publisher's version, 365 KB
Streszczenie w języku polskimFinal publisher's version, 404 KB
AcknowledgementsFinal publisher's version, 262 KB
Complete thesisFinal publisher's version, 11,5 MB
PropositionsFinal publisher's version, 19,2 KB

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title = "Insights into the transport mechanism of energy-coupling factor transporters",

abstract = "Vitamins are essential nutrients for organisms. Many organisms depend on cofactors obtained from the environment. Some organisms didn{\textquoteright}t develop complete biosynthetic pathways to produce those compounds or while having complete pathway they still have systems to scavenge nutrients from the extracellular environment. A selective uptake of nutrients is entrusted to transport proteins residing in the cell membrane. The importance of nutrient transport systems is reflected in high content of transport proteins in prokaryotic genomes (up to 16%). Transporters are proteins mostly specialized for a specific substrate or a group of substrates. The mechanisms of transport of key compounds for the bacterial survival may differ significantly. It is extremely interesting how different solutions evolved in nature to transport diverse solutes. Our work is concentrated around Energy-coupling factor (ECF) transporters. Those unique transporters use energy of ATP to transport vitamins and micronutrients across the prokaryotic lipid bilayer.",

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T1 - Insights into the transport mechanism of energy-coupling factor transporters

AU - Stanek, Weronika Karolina

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N2 - Vitamins are essential nutrients for organisms. Many organisms depend on cofactors obtained from the environment. Some organisms didn’t develop complete biosynthetic pathways to produce those compounds or while having complete pathway they still have systems to scavenge nutrients from the extracellular environment. A selective uptake of nutrients is entrusted to transport proteins residing in the cell membrane. The importance of nutrient transport systems is reflected in high content of transport proteins in prokaryotic genomes (up to 16%). Transporters are proteins mostly specialized for a specific substrate or a group of substrates. The mechanisms of transport of key compounds for the bacterial survival may differ significantly. It is extremely interesting how different solutions evolved in nature to transport diverse solutes. Our work is concentrated around Energy-coupling factor (ECF) transporters. Those unique transporters use energy of ATP to transport vitamins and micronutrients across the prokaryotic lipid bilayer.

AB - Vitamins are essential nutrients for organisms. Many organisms depend on cofactors obtained from the environment. Some organisms didn’t develop complete biosynthetic pathways to produce those compounds or while having complete pathway they still have systems to scavenge nutrients from the extracellular environment. A selective uptake of nutrients is entrusted to transport proteins residing in the cell membrane. The importance of nutrient transport systems is reflected in high content of transport proteins in prokaryotic genomes (up to 16%). Transporters are proteins mostly specialized for a specific substrate or a group of substrates. The mechanisms of transport of key compounds for the bacterial survival may differ significantly. It is extremely interesting how different solutions evolved in nature to transport diverse solutes. Our work is concentrated around Energy-coupling factor (ECF) transporters. Those unique transporters use energy of ATP to transport vitamins and micronutrients across the prokaryotic lipid bilayer.

M3 - Thesis fully internal (DIV)

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