@article{02a2f61539ff45dba53733817e29e746,
title = "Interaction of two strongly divergent archaellins stabilizes the structure of the Halorubrum archaellum",
abstract = "Halophilic archaea from the genus Halorubrum possess two extraordinarily diverged archaellin genes, flaB1 and flaB2. To clarify roles for each archaellin, we compared two natural Halorubrum lacusprofundi strains: One of them contains both archaellin genes, and the other has the flaB2 gene only. Both strains synthesize functional archaella; however, the strain, where both archaellins are present, is more motile. In addition, we expressed these archaellins in a Haloferax volcanii strain from which the endogenous archaellin genes were deleted. Three Hfx. volcanii strains expressing Hrr. lacusprofundi archaellins produced functional filaments consisting of only one (FlaB1 or FlaB2) or both (FlaB1/FlaB2) archaellins. All three strains were motile, although there were profound differences in the efficiency of motility. Both native and recombinant FlaB1/FlaB2 filaments have greater thermal stability and resistance to low salinity stress than single-component filaments. Functional supercoiled Hrr. lacusprofundi archaella can be composed of either single archaellin: FlaB2 or FlaB1; however, the two divergent archaellin subunits provide additional stabilization to the archaellum structure and thus adaptation to a wider range of external conditions. Comparative genomic analysis suggests that the described combination of divergent archaellins is not restricted to Hrr. lacusprofundi, but is occurring also in organisms from other haloarchaeal genera.",
keywords = "archaea, archaellin, archaellum, Haloferax, Halorubrum, motility",
author = "Pyatibratov, {Mikhail G.} and Syutkin, {Alexey S.} and Quax, {Tessa E. F.} and Melnik, {Tatjana N.} and Papke, {R. Thane} and Gogarten, {Johann Peter} and Kireev, {Igor I.} and Surin, {Alexey K.} and Beznosov, {Sergei N.} and Galeva, {Anna V.} and Fedorov, {Oleg V.}",
note = "Funding Information: Russian Foundation for Basic Research, Grant/Award Number: 19-04-00613 A; RFBR, Grant/Award Number: 19-04-01327 A; Emmy Noether grant from the Deutsche Forschungs Gemeinschaft, Grant/Award Number: 411069969; MSU Development Program, Grant/Award Number: PNR 5.13; and United Pushchino Center ?Structural and functional studies of proteins and RNA,? Grant/Award Number: 584307. This work was supported by a RFBR grant No. 19-04-00613 A to M.P., an Emmy Noether grant (411069969) from the Deutsche Forschungs Gemeinschaft to T.E.F.Q. and in part by a RFBR grant No. 19-04-01327 A to A.S. We thank Dr. T. Allers for pTA1228 plasmid, Dr. R. Cavicchioli and Dr. M. Pohlschr?der for the kindly presented strains, and M. Suvorina for the help with MS analysis. Electron microscopy was performed with the support of the Moscow State University development program (PNR 5.13). The mass spectrometric analysis was performed in facilities of the United Pushchino Center ?Structural and functional studies of proteins and RNA? (584307). Funding Information: Russian Foundation for Basic Research, Grant/Award Number: 19‐04‐00613 A; RFBR, Grant/Award Number: 19‐04‐01327 A; Emmy Noether grant from the Deutsche Forschungs Gemeinschaft, Grant/Award Number: 411069969; MSU Development Program, Grant/Award Number: PNR 5.13; and United Pushchino Center “Structural and functional studies of proteins and RNA,” Grant/Award Number: 584307. Funding Information: This work was supported by a RFBR grant No. 19‐04‐00613 A to M.P., an Emmy Noether grant (411069969) from the Deutsche Forschungs Gemeinschaft to T.E.F.Q. and in part by a RFBR grant No. 19‐04‐01327 A to A.S. We thank Dr. T. Allers for pTA1228 plasmid, Dr. R. Cavicchioli and Dr. M. Pohlschr{\"o}der for the kindly presented strains, and M. Suvorina for the help with MS analysis. Electron microscopy was performed with the support of the Moscow State University development program (PNR 5.13). The mass spectrometric analysis was performed in facilities of the United Pushchino Center “Structural and functional studies of proteins and RNA” (584307). Publisher Copyright: {\textcopyright} 2020 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.",
year = "2020",
month = jul,
doi = "10.1002/mbo3.1047",
language = "English",
volume = "9",
journal = "MicrobiologyOpen",
issn = "2045-8827",
publisher = "Wiley",
number = "7",
}