Intrachanges as part of complex chromosome-type exchange aberrations

JJWA Boei*, S Vermeulen, J Moser, LHF Mullenders, AT Natarajan

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

14 Citaten (Scopus)

Samenvatting

The chromosome-type exchange aberrations induced by ionizing radiation during the G(0)/G(1) phase of the cell cycle are believed to be the result of illegitimate rejoining of chromosome breaks. From numerous studies using chromosome painting, it has emerged that even after a moderate dose of radiation, a substantial fraction of these exchanges is complex. Most of them are derived from the free interaction between the ends of three or more breaks. Other studies have demonstrated that chromosomes occupy distinct territories in the interphase nucleus. Since breaks that are in close proximity have an enhanced interaction probability, it seems likely that after ionizing radiation many of the interacting breaks will be present within one chromosome or chromosome arm. Unfortunately, the majority of these intrachanges remain undetected, even when sophisticated molecular cytogenetic detection methods (i.e. mFISH) are applied to paint all chromosome pairs in distinct colors. In the present paper, we evaluate the limitations of full-color painting for the detection of complex exchanges and the correct interpretations of break interactions. (C) 2002 Elsevier Science B.V. All rights reserved.

Originele taal-2English
Pagina's (van-tot)47-55
Aantal pagina's9
TijdschriftMutation research-Fundamental and molecular mechanisms of mutagenesis
Volume504
Nummer van het tijdschrift1-2
DOI's
StatusPublished - 25-jul-2002
Evenement5th International Symposium on Chromosomal Aberrations/20th Meeting of the Japanese EMS Mammalian Mutagenicity Study Group - AWAJI ISL, Japan
Duur: 26-okt-200128-okt-2001

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