Isolation of Microglia and Immune Infiltrates from Mouse and Primate Central Nervous System

Thais F. Galatro, Ilia D. Vainchtein, Nieske Brouwer, Erik W. G. M. Boddeke, Bart J. L. Eggen*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

16 Citaten (Scopus)

Samenvatting

Microglia are the innate immune cells of the central nervous system (CNS) and play an important role in the maintenance of tissue homeostasis, providing neural support and neuroprotection. Microglia constantly survey their environment and quickly respond to homeostatic perturbations. Microglia are increasingly implicated in neuropathological and neurodegenerative conditions, such as Alzheimer’s disease, Parkinson’s disease, and glioma progression. Here, we describe a detailed isolation protocol for microglia and immune infiltrates, optimized for large amounts of post mortem tissue from human and rhesus macaque, as well as smaller tissue amounts from mouse brain and spinal cord, that yield a highly purified microglia population (up to 98 % purity). This acute isolation protocol is based on mechanical dissociation and a two-step density gradient purification, followed by fluorescence-activated cell sorting (FACS) to obtain pure microglia and immune infiltrate populations.

Originele taal-2English
TitelInflammation. Methods and Protocols
RedacteurenBjörn E. Clausen, Jon D. Laman
UitgeverijHumana Press
Pagina's333-342
Aantal pagina's10
ISBN van elektronische versie978-1-4939-6786-5
ISBN van geprinte versie978-1-4939-6784-1
DOI's
StatusPublished - 1-jan-2017

Publicatie series

NaamMethods in Molecular Biology
Volume1559
ISSN van geprinte versie1064-3745

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