Laboratory diagnosis of paraneoplastic pemphigus

A. M. Poot*, G. F. H. Diercks, D. Kramer, I. Schepens, G. Klunder, T. Hashimoto, L. Borradori, M. F. Jonkman, H. H. Pas

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

66 Citaten (Scopus)

Samenvatting

BackgroundParaneoplastic pemphigus (PNP) is a multiorgan disease characterized by antibodies against plakins, desmogleins and the 2-macroglobulin-like-1 (A2ML1) protein, in association with an underlying neoplasm. Accurate diagnosis relies on the demonstration of these autoantibodies in serum.

ObjectivesTo evaluate the value of different laboratory techniques in the serological diagnosis of PNP.

MethodsWe performed immunoblotting, envoplakin (EP) enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence (IIF) on rat bladder, radioactive immunoprecipitation and a nonradioactive combined immunoprecipitation-immunoblot assay. Additional assays included BP180 ELISA and BP230 ELISA. We included the sera of 19 patients with PNP and 40 control subjects.

ResultsThe sensitivities were 63% for anti-EP ELISA, 74% for rat bladder IIF, 89% for immunoblotting, 95% for radioactive immunoprecipitation and 100% for nonradioactive immunoprecipitation. Specificities ranged from 86% to 100%. The BP180 and BP230 ELISAs had low sensitivity and specificity for PNP. The combination of rat bladder IIF and immunoblot showed 100% sensitivity and specificity. The analysis of sequential PNP sera showed that antibody titres may decrease over time, possibly resulting in negative outcomes for EP ELISA and rat bladder IIF studies.

ConclusionsThe detection of autoantibodies against EP and periplakin, or A2ML1 by immunoprecipitation is most sensitive for PNP. The combination of rat bladder IIF and immunoblotting is equally sensitive and highly specific, and represents an alternative valuable and relatively easy approach for the serological diagnosis of PNP.

Originele taal-2English
Pagina's (van-tot)1016-1024
Aantal pagina's9
TijdschriftBRITISH JOURNAL OF DERMATOLOGY
Volume169
Nummer van het tijdschrift5
DOI's
StatusPublished - nov-2013

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