Lack of NMDA receptor subunit exchange alters Purkinje cell dendritic morphology in cerebellar slice cultures

F Metzger; Isabelle Pieri; U L M Eisel

doi:10.1016/j.devbrainres.2005.01.002

Lack of NMDA receptor subunit exchange alters Purkinje cell dendritic morphology in cerebellar slice cultures

F Metzger^*
, Isabelle Pieri
, U L M Eisel

^*Corresponding author voor dit werk

Eisel lab - Molecular Neurobiology and Neuroimmunology

Onderzoeksoutput: Article › Academic › peer review

5 Citaten (Scopus)

362 Downloads (Pure)

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Early postnatal developmental changes in N-methyl-D-aspartate (NMDA) receptor (NR) subunits regulate cerebellar granule cell maturation and potentially Purkinje cell development. We therefore investigated Purkinje cell morphology in slice cultures from mice with genetic subunit exchange from NR2C to NP2B (NRX-2B). NR2C-2B Purkinje cells after 12 days in vitro showed a significantly impaired dendritic arbour complexity with reduced branching density as compared to wild-type cells, a phenotype that was reversed by NMDA treatment. These data support the concept that in cerebellar slice cultures, Purkinje cell dendritic outgrowth is regulated by granule cell inputs. (c) 2005 Elsevier B.V. All rights reserved.

Originele taal-2	English
Pagina's (van-tot)	165-168
Aantal pagina's	4
Tijdschrift	Developmental Brain Research
Volume	155
Nummer van het tijdschrift	2
DOI's	https://doi.org/10.1016/j.devbrainres.2005.01.002
Status	Published - 31-mrt.-2005

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@article{e822491bf26246aeb592e9a28299eb10,

title = "Lack of NMDA receptor subunit exchange alters Purkinje cell dendritic morphology in cerebellar slice cultures",

abstract = "Early postnatal developmental changes in N-methyl-D-aspartate (NMDA) receptor (NR) subunits regulate cerebellar granule cell maturation and potentially Purkinje cell development. We therefore investigated Purkinje cell morphology in slice cultures from mice with genetic subunit exchange from NR2C to NP2B (NRX-2B). NR2C-2B Purkinje cells after 12 days in vitro showed a significantly impaired dendritic arbour complexity with reduced branching density as compared to wild-type cells, a phenotype that was reversed by NMDA treatment. These data support the concept that in cerebellar slice cultures, Purkinje cell dendritic outgrowth is regulated by granule cell inputs. (c) 2005 Elsevier B.V. All rights reserved.",

keywords = "NMDA-NR2 subunits, organotypic slice cultures, dendritic differentiation, cerebellar granule cells, Purkinje cells, PROTEIN-KINASE-C, IN-VITRO, GROWTH, BRAIN, DIFFERENTIATION, EXPRESSION, MICE",

author = "F Metzger and Isabelle Pieri and Eisel, \{U L M\}",

year = "2005",

month = mar,

day = "31",

doi = "10.1016/j.devbrainres.2005.01.002",

language = "English",

volume = "155",

pages = "165--168",

journal = "Developmental Brain Research",

issn = "0165-3806",

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TY - JOUR

T1 - Lack of NMDA receptor subunit exchange alters Purkinje cell dendritic morphology in cerebellar slice cultures

AU - Metzger, F

AU - Pieri, Isabelle

AU - Eisel, U L M

PY - 2005/3/31

Y1 - 2005/3/31

N2 - Early postnatal developmental changes in N-methyl-D-aspartate (NMDA) receptor (NR) subunits regulate cerebellar granule cell maturation and potentially Purkinje cell development. We therefore investigated Purkinje cell morphology in slice cultures from mice with genetic subunit exchange from NR2C to NP2B (NRX-2B). NR2C-2B Purkinje cells after 12 days in vitro showed a significantly impaired dendritic arbour complexity with reduced branching density as compared to wild-type cells, a phenotype that was reversed by NMDA treatment. These data support the concept that in cerebellar slice cultures, Purkinje cell dendritic outgrowth is regulated by granule cell inputs. (c) 2005 Elsevier B.V. All rights reserved.

AB - Early postnatal developmental changes in N-methyl-D-aspartate (NMDA) receptor (NR) subunits regulate cerebellar granule cell maturation and potentially Purkinje cell development. We therefore investigated Purkinje cell morphology in slice cultures from mice with genetic subunit exchange from NR2C to NP2B (NRX-2B). NR2C-2B Purkinje cells after 12 days in vitro showed a significantly impaired dendritic arbour complexity with reduced branching density as compared to wild-type cells, a phenotype that was reversed by NMDA treatment. These data support the concept that in cerebellar slice cultures, Purkinje cell dendritic outgrowth is regulated by granule cell inputs. (c) 2005 Elsevier B.V. All rights reserved.

KW - NMDA-NR2 subunits

KW - organotypic slice cultures

KW - dendritic differentiation

KW - cerebellar granule cells

KW - Purkinje cells

KW - PROTEIN-KINASE-C

KW - IN-VITRO

KW - GROWTH

KW - BRAIN

KW - DIFFERENTIATION

KW - EXPRESSION

KW - MICE

U2 - 10.1016/j.devbrainres.2005.01.002

DO - 10.1016/j.devbrainres.2005.01.002

M3 - Article

SN - 0165-3806

VL - 155

SP - 165

EP - 168

JO - Developmental Brain Research

JF - Developmental Brain Research

IS - 2

ER -

Lack of NMDA receptor subunit exchange alters Purkinje cell dendritic morphology in cerebellar slice cultures

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