LED-phototherapy does not induce oxidative DNA damage in hyperbilirubinemic Gunn rats

Lori W E van der Schoor; Christian V Hulzebos; Martijn H van Faassen; Ido P Kema; Alain de Bruin; Rick Havinga; Mirjam Koster; Sameh A Youssef; Laura Bongiovanni; Johan W Jonker; Henkjan J Verkade

doi:10.1038/s41390-019-0367-y

LED-phototherapy does not induce oxidative DNA damage in hyperbilirubinemic Gunn rats

Lori W E van der Schoor, Christian V Hulzebos, Martijn H van Faassen, Ido P Kema, Alain de Bruin, Rick Havinga, Mirjam Koster, Sameh A Youssef, Laura Bongiovanni, Johan W Jonker, Henkjan J Verkade

Onderzoeksoutput › Academic › peer review

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BACKGROUND: Phototherapy (PT) is the standard treatment of neonatal unconjugated hyperbilirubinemia. Fluorescent tube (FT)-emitted PT light is known to induce oxidative DNA damage in neonates. Nowadays, however, FTs have largely been replaced by light-emitting diodes (LEDs) for delivering PT. Until now, it is unknown whether LED-PT causes oxidative DNA damage. We aim to determine whether LED-PT induces oxidative DNA damage in hyperbilirubinemic rats.

METHODS: Adult Gunn rats, with genetically unconjugated hyperbilirubinemia, received LED-PT in the clinically relevant doses of 10 or 30 mu W/cm(2)/nm. Urine was collected at 0, 24, and 48 h of PT. A group of young Gunn rats received intensive LED-PT of 100 mu W/cm(2)/nm for 24 h. Urine was collected every 8 h and analyzed for the levels of oxidative DNA damage marker 8-hydroxy-2'deoxyguanosine (8-OHdG) and creatinine. DNA damage was evaluated by immunohistochemistry (gamma H2AX) of skin and spleen samples.

RESULTS: LED-PT of 10 and 30 mu W/cm(2)/nm did not affect urinary concentrations of 8-OHdG and creatinine or the 8-OHdG/creatinine ratio. Likewise, intensive LED-PT did not affect the 8-OHdG/creatinine ratio or the number of gamma H2AX-positive cells in the skin or spleen.

CONCLUSIONS: Our results show that LED-PT does not induce oxidative DNA damage in hyperbilirubinemic Gunn rats either at clinically relevant or intensive dosages.

Originele taal-2	English
Pagina's (van-tot)	1041-1047
Aantal pagina's	7
Tijdschrift	Pediatric Research
Volume	85
Nummer van het tijdschrift	7
DOI's	https://doi.org/10.1038/s41390-019-0367-y
Status	Published - jun.-2019

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LED-phototherapy does not induce oxidative DNA damage in hyperbilirubinemic Gunn ratsFinal publisher's version, 1,75 MBLicentie: Taverne

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@article{79fb77c886dc4df7aec1dc459f9ba68f,

title = "LED-phototherapy does not induce oxidative DNA damage in hyperbilirubinemic Gunn rats",

abstract = "BACKGROUND: Phototherapy (PT) is the standard treatment of neonatal unconjugated hyperbilirubinemia. Fluorescent tube (FT)-emitted PT light is known to induce oxidative DNA damage in neonates. Nowadays, however, FTs have largely been replaced by light-emitting diodes (LEDs) for delivering PT. Until now, it is unknown whether LED-PT causes oxidative DNA damage. We aim to determine whether LED-PT induces oxidative DNA damage in hyperbilirubinemic rats.METHODS: Adult Gunn rats, with genetically unconjugated hyperbilirubinemia, received LED-PT in the clinically relevant doses of 10 or 30 mu W/cm(2)/nm. Urine was collected at 0, 24, and 48 h of PT. A group of young Gunn rats received intensive LED-PT of 100 mu W/cm(2)/nm for 24 h. Urine was collected every 8 h and analyzed for the levels of oxidative DNA damage marker 8-hydroxy-2'deoxyguanosine (8-OHdG) and creatinine. DNA damage was evaluated by immunohistochemistry (gamma H2AX) of skin and spleen samples.RESULTS: LED-PT of 10 and 30 mu W/cm(2)/nm did not affect urinary concentrations of 8-OHdG and creatinine or the 8-OHdG/creatinine ratio. Likewise, intensive LED-PT did not affect the 8-OHdG/creatinine ratio or the number of gamma H2AX-positive cells in the skin or spleen.CONCLUSIONS: Our results show that LED-PT does not induce oxidative DNA damage in hyperbilirubinemic Gunn rats either at clinically relevant or intensive dosages.",

keywords = "LIGHT-EMITTING-DIODES, RISK-FACTOR, STRESS, BILIRUBIN, PREVENTION, OXIDANT",

author = "{van der Schoor}, {Lori W E} and Hulzebos, {Christian V} and {van Faassen}, {Martijn H} and Kema, {Ido P} and {de Bruin}, Alain and Rick Havinga and Mirjam Koster and Youssef, {Sameh A} and Laura Bongiovanni and Jonker, {Johan W} and Verkade, {Henkjan J}",

year = "2019",

month = jun,

doi = "10.1038/s41390-019-0367-y",

language = "English",

volume = "85",

pages = "1041--1047",

journal = "Pediatric Research",

issn = "0031-3998",

publisher = "Nature Publishing Group",

number = "7",

}

TY - JOUR

T1 - LED-phototherapy does not induce oxidative DNA damage in hyperbilirubinemic Gunn rats

AU - van der Schoor, Lori W E

AU - Hulzebos, Christian V

AU - van Faassen, Martijn H

AU - Kema, Ido P

AU - de Bruin, Alain

AU - Havinga, Rick

AU - Koster, Mirjam

AU - Youssef, Sameh A

AU - Bongiovanni, Laura

AU - Jonker, Johan W

AU - Verkade, Henkjan J

PY - 2019/6

Y1 - 2019/6

N2 - BACKGROUND: Phototherapy (PT) is the standard treatment of neonatal unconjugated hyperbilirubinemia. Fluorescent tube (FT)-emitted PT light is known to induce oxidative DNA damage in neonates. Nowadays, however, FTs have largely been replaced by light-emitting diodes (LEDs) for delivering PT. Until now, it is unknown whether LED-PT causes oxidative DNA damage. We aim to determine whether LED-PT induces oxidative DNA damage in hyperbilirubinemic rats.METHODS: Adult Gunn rats, with genetically unconjugated hyperbilirubinemia, received LED-PT in the clinically relevant doses of 10 or 30 mu W/cm(2)/nm. Urine was collected at 0, 24, and 48 h of PT. A group of young Gunn rats received intensive LED-PT of 100 mu W/cm(2)/nm for 24 h. Urine was collected every 8 h and analyzed for the levels of oxidative DNA damage marker 8-hydroxy-2'deoxyguanosine (8-OHdG) and creatinine. DNA damage was evaluated by immunohistochemistry (gamma H2AX) of skin and spleen samples.RESULTS: LED-PT of 10 and 30 mu W/cm(2)/nm did not affect urinary concentrations of 8-OHdG and creatinine or the 8-OHdG/creatinine ratio. Likewise, intensive LED-PT did not affect the 8-OHdG/creatinine ratio or the number of gamma H2AX-positive cells in the skin or spleen.CONCLUSIONS: Our results show that LED-PT does not induce oxidative DNA damage in hyperbilirubinemic Gunn rats either at clinically relevant or intensive dosages.

AB - BACKGROUND: Phototherapy (PT) is the standard treatment of neonatal unconjugated hyperbilirubinemia. Fluorescent tube (FT)-emitted PT light is known to induce oxidative DNA damage in neonates. Nowadays, however, FTs have largely been replaced by light-emitting diodes (LEDs) for delivering PT. Until now, it is unknown whether LED-PT causes oxidative DNA damage. We aim to determine whether LED-PT induces oxidative DNA damage in hyperbilirubinemic rats.METHODS: Adult Gunn rats, with genetically unconjugated hyperbilirubinemia, received LED-PT in the clinically relevant doses of 10 or 30 mu W/cm(2)/nm. Urine was collected at 0, 24, and 48 h of PT. A group of young Gunn rats received intensive LED-PT of 100 mu W/cm(2)/nm for 24 h. Urine was collected every 8 h and analyzed for the levels of oxidative DNA damage marker 8-hydroxy-2'deoxyguanosine (8-OHdG) and creatinine. DNA damage was evaluated by immunohistochemistry (gamma H2AX) of skin and spleen samples.RESULTS: LED-PT of 10 and 30 mu W/cm(2)/nm did not affect urinary concentrations of 8-OHdG and creatinine or the 8-OHdG/creatinine ratio. Likewise, intensive LED-PT did not affect the 8-OHdG/creatinine ratio or the number of gamma H2AX-positive cells in the skin or spleen.CONCLUSIONS: Our results show that LED-PT does not induce oxidative DNA damage in hyperbilirubinemic Gunn rats either at clinically relevant or intensive dosages.

KW - LIGHT-EMITTING-DIODES

KW - RISK-FACTOR

KW - STRESS

KW - BILIRUBIN

KW - PREVENTION

KW - OXIDANT

U2 - 10.1038/s41390-019-0367-y

DO - 10.1038/s41390-019-0367-y

M3 - Article

C2 - 30851724

SN - 0031-3998

VL - 85

SP - 1041

EP - 1047

JO - Pediatric Research

JF - Pediatric Research

IS - 7

ER -

LED-phototherapy does not induce oxidative DNA damage in hyperbilirubinemic Gunn rats

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