Liver transcriptomic and methylomic analyses identify transcriptional mitogen-activated protein kinase regulation in facultative hibernation of Syrian hamster

Louis Coussement, Marloes M Oosterhof, Victor Guryev, Vera A Reitsema, Jojanneke J Bruintjes, Maaike Goris, Hjalmar R Bouma, Tim de Meyer, Marianne G Rots, Robert H Henning*

*Bijbehorende auteur voor dit werk

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Hibernation consists of alternating torpor-arousal phases, during which animals cope with repetitive hypothermia and ischaemia-reperfusion. Due to limited transcriptomic and methylomic information for facultative hibernators, we here conducted RNA and whole-genome bisulfide sequencing in liver of hibernating Syrian hamster ( Mesocricetus auratus). Gene ontology analysis was performed on 844 differentially expressed genes and confirmed the shift in metabolic fuel utilization, inhibition of RNA transcription and cell cycle regulation as found in seasonal hibernators. Additionally, we showed a so far unreported suppression of mitogen-activated protein kinase (MAPK) and protein phosphatase 1 pathways during torpor. Notably, hibernating hamsters showed upregulation of MAPK inhibitors (dual-specificity phosphatases and sproutys) and reduced levels of MAPK-induced transcription factors (TFs). Promoter methylation was found to modulate the expression of genes targeted by these TFs. In conclusion, we document gene regulation between hibernation phases, which may aid the identification of pathways and targets to prevent organ damage in transplantation or ischaemia-reperfusion.

Originele taal-2English
Artikelnummer20230368
Aantal pagina's11
TijdschriftProceedings. Biological sciences
Volume290
Nummer van het tijdschrift1999
DOI's
StatusPublished - 24-mei-2023

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