Localization at a subband level of polymorphic 13q14 DNA probes for diagnosis of hereditary retinoblastoma and Wilson disease

Hans Scheffer; Ido P. Kema; Ikuko Kondo; Anneke Y van der Veen; Tatsuro Ikeuchi; Charles H. C. M.  Buys

doi:10.1007/BF00291421

Localization at a subband level of polymorphic 13q14 DNA probes for diagnosis of hereditary retinoblastoma and Wilson disease

Hans Scheffer, Ido P. Kema, Ikuko Kondo, Anneke Y van der Veen, Tatsuro Ikeuchi, Charles H. C. M. Buys

Onderzoeksoutput: Article › Academic › peer review

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Two single-copy DNA sequences, pG24E6.8 (D13S21) detecting a low-frequency MspI RFLP and pG14E1.9 (D13S22) detecting a high-frequency DraI RFLP, have been isolated and cloned from a human chromosome 13-specific phage library and localized at 13q14. Their subband localization was described using a panel of cell lines from patients with different chromosome 13 deletions. A quantitative analysis of hybridization signals was carried out, taking for reference a single-copy DNA sequence from another chromosome. D13S21 and D13S22 were both assigned to q14.1-14.2, which also harbors the genes responsible for retinoblastoma and Wilson disease. The DraI polymorphism detected by pG14E1.9 is a very suitable one for linkage studies in families with either disease

Originele taal-2	English
Pagina's (van-tot)	335-337
Aantal pagina's	3
Tijdschrift	HUMAN GENETICS
Volume	77
Nummer van het tijdschrift	4
DOI's	https://doi.org/10.1007/BF00291421
Status	Published - dec.-1987

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title = "Localization at a subband level of polymorphic 13q14 DNA probes for diagnosis of hereditary retinoblastoma and Wilson disease",

abstract = "Two single-copy DNA sequences, pG24E6.8 (D13S21) detecting a low-frequency MspI RFLP and pG14E1.9 (D13S22) detecting a high-frequency DraI RFLP, have been isolated and cloned from a human chromosome 13-specific phage library and localized at 13q14. Their subband localization was described using a panel of cell lines from patients with different chromosome 13 deletions. A quantitative analysis of hybridization signals was carried out, taking for reference a single-copy DNA sequence from another chromosome. D13S21 and D13S22 were both assigned to q14.1-14.2, which also harbors the genes responsible for retinoblastoma and Wilson disease. The DraI polymorphism detected by pG14E1.9 is a very suitable one for linkage studies in families with either disease",

author = "Hans Scheffer and Kema, {Ido P.} and Ikuko Kondo and {van der Veen}, {Anneke Y} and Tatsuro Ikeuchi and Buys, {Charles H. C. M.}",

year = "1987",

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doi = "10.1007/BF00291421",

language = "English",

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TY - JOUR

T1 - Localization at a subband level of polymorphic 13q14 DNA probes for diagnosis of hereditary retinoblastoma and Wilson disease

AU - Scheffer, Hans

AU - Kema, Ido P.

AU - Kondo, Ikuko

AU - van der Veen, Anneke Y

AU - Ikeuchi, Tatsuro

AU - Buys, Charles H. C. M.

PY - 1987/12

Y1 - 1987/12

N2 - Two single-copy DNA sequences, pG24E6.8 (D13S21) detecting a low-frequency MspI RFLP and pG14E1.9 (D13S22) detecting a high-frequency DraI RFLP, have been isolated and cloned from a human chromosome 13-specific phage library and localized at 13q14. Their subband localization was described using a panel of cell lines from patients with different chromosome 13 deletions. A quantitative analysis of hybridization signals was carried out, taking for reference a single-copy DNA sequence from another chromosome. D13S21 and D13S22 were both assigned to q14.1-14.2, which also harbors the genes responsible for retinoblastoma and Wilson disease. The DraI polymorphism detected by pG14E1.9 is a very suitable one for linkage studies in families with either disease

AB - Two single-copy DNA sequences, pG24E6.8 (D13S21) detecting a low-frequency MspI RFLP and pG14E1.9 (D13S22) detecting a high-frequency DraI RFLP, have been isolated and cloned from a human chromosome 13-specific phage library and localized at 13q14. Their subband localization was described using a panel of cell lines from patients with different chromosome 13 deletions. A quantitative analysis of hybridization signals was carried out, taking for reference a single-copy DNA sequence from another chromosome. D13S21 and D13S22 were both assigned to q14.1-14.2, which also harbors the genes responsible for retinoblastoma and Wilson disease. The DraI polymorphism detected by pG14E1.9 is a very suitable one for linkage studies in families with either disease

U2 - 10.1007/BF00291421

DO - 10.1007/BF00291421

M3 - Article

SN - 0340-6717

VL - 77

SP - 335

EP - 337

JO - HUMAN GENETICS

JF - HUMAN GENETICS

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