Macromolecular Crowding Measurements with Genetically Encoded Probes Based on Förster Resonance Energy Transfer in Living Cells

Sara Mouton, Liesbeth M. Veenhoff, Arnold J. Boersma

OnderzoeksoutputAcademicpeer review

Samenvatting

Genetically encoded Förster resonance energy transfer (FRET)-based probes allow a sensitive readout for different or specific parameters in the living cell. We previously demonstrated how FRET-based probes could quantify macromolecular crowding with high spatio-temporal resolution and under various conditions. Here, we present a protocol developed for the use of FRET-based crowding probes in baker’s yeast, but the general considerations also apply to other species, as well as other FRET-based sensors. This method allows straightforward detection of macromolecular crowding under challenging conditions often presented by living cells.

Originele taal-2English
Pagina's (van-tot)169-180
Aantal pagina's11
TijdschriftMethods in Molecular Biology
Volume2175
DOI's
StatusPublished - 2020

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