Majority of cerebrospinal fluid-contacting neurons in the spinal cord of C57Bl/6 N mice is present in ectopic position unlike in other studied experimental mice strains and mammalian species

Zuzana Tonelli Gombalová, Ján Košuth, Anna Alexovič Matiašová, Jarmila Zrubáková, Ivan Žežula, Toniella Giallongo, Anna Maria Di Giulio, Stephana Carelli, Lenka Tomašková, Zuzana Daxnerová, Juraj Ševc*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

6 Citaten (Scopus)


Cerebrospinal fluid contacting neurons (CSF-cNs) represent a specific class of neurons located in close vicinity of brain ventricles and central canal. In contrast with knowledge gained from other vertebrate species, we found that vast majority of CSF-cNs in the spinal cord of C57Bl/6N mice is located in ectopic distal ventral position. However, we found that small number of ectopic CSF-cNs is present also in spinal cord of other investigated experimental mice strains (C57Bl/6J, Balb/C) and mammalian species (Wistar rats, New Zealand White rabbits). Similarly, as the proximal populations, ectopic CSF-cNs retain PKD2L1-immunoreactivity and synaptic contacts with other neurons. On the other side, they show rather multipolar morphology lacking thick dendrite contacting central canal lumen. Ectopic CSF-cNs in the spinal cord of C57Bl/6N mice emerge during whole period devoted to production of CSF-cNs and reach their ventral destinations during first postnatal weeks. In order to identify major gene, whose impairment could trigger translocation of CSF-cNs outside the central canal area, we took advantage of close consanguinity of C57Bl/6J substrain with normal CSF-cN distribution and C57Bl/6N substrain with majority of CSF-cNs in ectopic position. Employing in silico analyses, we ranked polymorphisms in C57Bl/6N substrain and selected genes Crb1, Cyfip2, Adamts12, Plk1, and Herpud2 as the most probable candidates, whose product dysfunction might be responsible for the ectopic distribution of CSF-cNs. Furthermore, segregation analysis of F2 progeny of parental C57Bl/6N and Balb/C mice revealed that polymorphic loci of Crb1 and Cyfip2 underlie the ectopic position of CSF-cNs in the spinal cord of C57Bl/6N mice.

Originele taal-2English
Pagina's (van-tot)2523-2550
Aantal pagina's28
TijdschriftThe Journal of Comparative Neurology
Nummer van het tijdschrift15
Vroegere onlinedatum25-mrt.-2020
StatusPublished - 15-okt.-2020
Extern gepubliceerdJa

Citeer dit