TY - JOUR
T1 - Mass spectrometry based approaches and strategies in bioanalysis for qualitative and quantitative analysis of pharmaceutically relevant molecules
AU - Klont, Frank
AU - Hopfgartner, Gérard
PY - 2021/12/13
Y1 - 2021/12/13
N2 - Mass spectrometry plays an essential role in qualitative and quantitative analysis of pharmaceutically relevant molecules. The present review summarizes some the most common applications of LC-MS for the characterization of therapeutic low-molecular-weight compounds, peptides and proteins, and oligonucleotides using low-resolution and high-resolution tandem mass spectrometry. In addition, the benefit of multistage MS, differential ion mobility, and data independent acquisition is emphasized. At last, the potential of coupling MS with novel interfaces for high-throughput analysis is discussed.
AB - Mass spectrometry plays an essential role in qualitative and quantitative analysis of pharmaceutically relevant molecules. The present review summarizes some the most common applications of LC-MS for the characterization of therapeutic low-molecular-weight compounds, peptides and proteins, and oligonucleotides using low-resolution and high-resolution tandem mass spectrometry. In addition, the benefit of multistage MS, differential ion mobility, and data independent acquisition is emphasized. At last, the potential of coupling MS with novel interfaces for high-throughput analysis is discussed.
U2 - 10.1016/j.ddtec.2021.10.004
DO - 10.1016/j.ddtec.2021.10.004
M3 - Review article
SN - 1740-6749
VL - 40
SP - 64
EP - 68
JO - Drug Discovery Today: Technologies
JF - Drug Discovery Today: Technologies
ER -