Samenvatting
The invention provides means and methods for efficiently cloning nucleic acid sequences of interest in micro-organisms that are less amenable to conventional nucleic acid manipulations, as compared to, for instance, E.coli. The present invention enables high-throughput cloning (and, preferably, expressing) of a nucleic acid of interest in a first kind of micro-organism, while an initial (preferably high throughput) cloning of the nucleic acid of interest is done in a second kind of micro-organism.
Originele taal-2 | English |
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Octrooinummer | WO2008147193 |
Prioriteitsdatum | 01/06/2007 |
Indieningsdatum | 30/05/2008 |
Status | Published - 4-dec.-2008 |