Measuring and Interpreting Nuclear Transport in Neurodegenerative Disease-The Example of C9orf72 ALS

Marije F W Semmelink, Anton Steen, Liesbeth M Veenhoff*

*Bijbehorende auteur voor dit werk

Onderzoeksoutputpeer review

7 Citaten (Scopus)
25 Downloads (Pure)

Samenvatting

Transport from and into the nucleus is essential to all eukaryotic life and occurs through the nuclear pore complex (NPC). There are a multitude of data supporting a role for nuclear transport in neurodegenerative diseases, but actual transport assays in disease models have provided diverse outcomes. In this review, we summarize how nuclear transport works, which transport assays are available, and what matters complicate the interpretation of their results. Taking a specific type of ALS caused by mutations in C9orf72 as an example, we illustrate these complications, and discuss how the current data do not firmly answer whether the kinetics of nucleocytoplasmic transport are altered. Answering this open question has far-reaching implications, because a positive answer would imply that widespread mislocalization of proteins occurs, far beyond the reported mislocalization of transport reporters, and specific proteins such as FUS, or TDP43, and thus presents a challenge for future research.

Originele taal-2English
Artikelnummer9217
Aantal pagina's21
TijdschriftInternational Journal of Molecular Sciences
Volume22
Nummer van het tijdschrift17
DOI's
StatusPublished - 26-aug.-2021

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