Mechanism-Inspired Engineering of Phenylalanine Aminomutase for Enhanced β-Regioselective Asymmetric Amination of Cinnamates

Bian Wu, Wiktor Szymanski, Gjalt G. Wybenga, Matthew M. Heberling, Sebastian Bartsch, Stefaan de Wildeman, Gerrit J. Poelarends, Ben L. Feringa, Bauke W. Dijkstra, Dick B. Janssen*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

44 Citaten (Scopus)

Samenvatting

Turn to switch: A mutant of phenylalanine aminomutase was engineered that can catalyze the regioselective amination of cinnamate derivatives to, for example, β-amino acids. This regioselectivity, along with the X-ray crystal structures, suggests two distinct carboxylate binding modes differentiated by Cβ-Cipso bond rotation, which determines if β- or α-addition takes place.
Originele taal-2English
Pagina's (van-tot)482-486
Aantal pagina's5
TijdschriftAngewandte Chemie-International Edition
Volume51
Nummer van het tijdschrift2
DOI's
StatusPublished - 2012

Citeer dit