Merging Whole-cell Biosynthesis of Styrene and Transition-metal Catalyzed Derivatization Reactions

Ruben V. Maaskant, Shreyans Chordia, Gerard Roelfes*

*Corresponding author voor dit werk

OnderzoeksoutputAcademicpeer review

15 Citaten (Scopus)
65 Downloads (Pure)


The approach of combining enzymatic and transition-metal catalysis has been focused almost exclusively on using purified, isolated enzymes. The use of whole-cell biocatalysis, instead of isolated enzymes, with transition-metal catalysis, however, has been investigated only sparsely to date. Herein we present the development of two transition-metal catalyzed reactions used to derivatize styrene obtained from whole-cell biosynthesis. Using a biocompatible ruthenium cross-metathesis catalyst up to 1.5 mM stilbene could be obtained in the presence of E. coli, which simultaneously produced styrene. Using palladium catalysts and arylboronic acids, titers of up to 1 mM of several stilbene derivatives were obtained. These two transition-metal catalyzed reactions are valuable additions to the toolbox of combined whole-cell biocatalysis and transition-metal catalysis, offering the possibility to supplement biosynthetic pathways with the chemical versatility of abiological transition-metal catalysis.

Originele taal-2English
Pagina's (van-tot)1607-1613
Aantal pagina's7
Nummer van het tijdschrift6
StatusPublished - 19-mrt.-2021


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