@inbook{83df7445d87a4bbeb448517269e12945,
title = "Methods to Analyze Motility in Eury- and Crenarchaea",
abstract = "Many archaea display swimming motility in liquid medium, which is empowered by the archaellum. Directional movement requires a functional archaellum and a sensing system, such as the chemotaxis system that is used by Euryarchaea. Two well-studied models are the euryarchaeon Haloferax volcanii and the crenarchaeon Sulfolobus acidocaldarius. In this chapter we describe two methods to analyze their swimming behavior and directional movement: (a) time-lapse microscopy under native temperatures and (b) spotting on semi-solid agar or gelrite plates. Whereas the first method allows for deep analysis of swimming behavior, the second method is suited for high throughput comparison of multiple strains.",
keywords = "Agar, Archaeal Proteins, Chemotaxis, Haloferax volcanii, Sulfolobus acidocaldarius",
author = "Megha Patro and {van Wolferen}, Marleen and Xing Ye and Sonja-Verena Albers and Quax, {Tessa E F}",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2445-6_25",
language = "English",
isbn = "978-1-0716-2444-9",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Springer",
pages = "373--385",
editor = "Ferreira-Cerca, {S{\'e}bastien }",
booktitle = "Archaea",
}