Mineralization of the herbicide 2,3,6-trichlorobenzoic acid by a co-culture of anaerobic and aerobic bacteria

Jan Gerritse*, Jan C. Gottschal

*Bijbehorende auteur voor dit werk

    OnderzoeksoutputAcademicpeer review

    46 Citaten (Scopus)


    Bacteria from an anaerobic enrichment reductively removed chlorine from the ortho- position of 2,3,6-trichlorobenzoic acid (2,3,6-TBA) producing 2,5-dichlorobenzoate (2,5-DBA). The strictly aerobic bacterium Pseudomonas aeruginosa JB2 subsequently used 2,5-DBA as a growth substrate in the presence of oxygen. The anaerobic dechlorinating microbial population was grown with P. aeruginosa JB2 in continuous culture. Inside the liquid culture, a nylon netting, on a stainless-steel support, contained vermiculite particles to provide a strictly anaerobic environment within the aerated culture. Complete mineralization of 2,3,6-TBA depended on the extent of oxygen input into the reactor. Under strictly anaerobic conditions 2,5-DBA and Cl- were produced stoichiometrically through the reductive dechlorination of 2,3,6-TBA. This process of reductive dechlorination was not inhibited by (moderate) aeration resulting in an O2-concentration of 0.3-0.5-mu-M in the culture liquid.

    Originele taal-2English
    Pagina's (van-tot)89-98
    Aantal pagina's10
    TijdschriftFEMS Microbial Ecology
    Nummer van het tijdschrift2
    StatusPublished - 1-jul-1992

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