@article{3a0c5483dfa94d55b05abd8c87cfdab0,
title = "Molecular Cloning with a pMEA300-Derived Shuttle Vector and Characterization of the Amycolatopsis methanolica Prephenate Dehydratase Gene",
abstract = "An efficient restriction barrier for methylated DNA in the actinomycete Amycolatopsis metanolica could be avoided by using a nonmethylating Escherichia coli strain for DNA isolations. The A. methanolica prephenate dehydratase gene was cloned from a gene bank in a pMEA300-derived shuttle vector in E. coli and characterized.",
keywords = "NUCLEOTIDE-SEQUENCE, STREPTOMYCES, RESTRICTION, PHENYLALANINE, PHEA, CONSTRUCTION, MUTAGENESIS, BACTERIA, SYSTEMS, PROTEIN",
author = "J.W. Vrijbloed and \{Hylckama Vlieg\}, \{J. van\} and Put, \{N.M.J. van der\} and G.I. Hessels and L. Dijkhuizen",
note = "Relation: http://www.rug.nl/gbb/ date\_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute",
year = "1995",
month = nov,
language = "English",
volume = "177",
pages = "6666--6669",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "AMER SOC MICROBIOLOGY",
number = "22",
}