Monitoring the Formation of Autophagosomal Precursor Structures in Yeast Saccharomyces cerevisiae

R. Gomez-Sanchez, J. Sanchez-Wandelmer, F. Reggiori*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

1 Citaat (Scopus)

Samenvatting

The budding yeast Saccharomyces cerevisiae is a powerful and versatile model organism for studying multiple aspects of the biology of eukaryotic cells, including the molecular principles underlying autophagy. One of the unique advantages of this unicellular system is its amenability to genetic and biochemical approaches, which had a pivotal role in the discovery and characterization of most of the autophagy-related (Atg) proteins, the central players of autophagy. The relevance of investigating autophagy in this cell model lies in the high conservation of this pathway among eukaryotes, i.e., most of the yeast Atg proteins possess one or more mammalian orthologs. In addition to the experimental advantages, a very large collection of reagents keeps S. cerevisiae in a leading position for the study of the molecular mechanism and regulation of autophagy. In this chapter, we describe fluorescence microscopy and biochemical methods that allow to monitor in vivo the assembly the of Atg machinery, a key step of autophagy. These approaches can be very useful to those researchers that would like to assess the progression of the autophagosomal precursor structure formation under various conditions, in the presence of specific Atg protein mutants or in the absence of other factors.

Originele taal-2English
TitelMolecular Characterization of Autophagic Responses, Part B
RedacteurenLorenzo Galluzzi, José Manuel Bravo-San Pedro, Guido Kroemer
UitgeverijAcademic Press
Pagina's323-365
Aantal pagina's43
Volume588
ISBN van elektronische versie9780128097946
ISBN van geprinte versie9780128096741
DOI's
StatusPublished - 22-feb-2017

Publicatie series

NaamMethods in Enzymology
UitgeverijELSEVIER ACADEMIC PRESS INC
Volume588
ISSN van geprinte versie0076-6879

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