Mouse Lung Tissue Slice Culture

Xinhui Wu, Eline M van Dijk, I Sophie T Bos, Loes E M Kistemaker, Reinoud Gosens

OnderzoeksoutputAcademicpeer review

2 Citaten (Scopus)

Samenvatting

Precision-cut lung slices (PCLS) represent an ex vivo model widely used in visualizing interactions between lung structure and function. The major advantage of this technique is that the presence, differentiation state, and localization of the more than 40 cell types that make up the lung are in accordance with the physiological situation found in lung tissue, including the right localization and patterning of extracellular matrix elements. Here we describe the methodology involved in preparing and culturing PCLS followed by detailed practical information about their possible applications.

Originele taal-2English
TitelMouse cell culture
Plaats van productieNew York
UitgeverijHumana Press
Pagina's297-311
Aantal pagina's15
ISBN van elektronische versie978-1-4939-9086-3
ISBN van geprinte versie978-1-4939-9085-6
DOI's
StatusPublished - 21-feb-2019

Publicatie series

NaamMethods in Molecular Biology
Volume1940
ISSN van geprinte versie1064-3745

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