mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines

Rubén Gómez-Sánchez, Sokhna M S Yakhine-Diop, Mario Rodríguez-Arribas, José M Bravo-San Pedro, Guadalupe Martínez-Chacón, Elisabet Uribe-Carretero, Diana C J Pinheiro de Castro, Elisa Pizarro-Estrella, José M Fuentes, Rosa A González-Polo

    OnderzoeksoutputAcademicpeer review

    46 Citaten (Scopus)
    351 Downloads (Pure)

    Samenvatting

    We characterized the dynamics of autophagy in vitro using four different cell systems and analyzing markers widely used in this field, i.e. LC3 (microtubule-associated protein 1 light chain 3; protein recruited from the cytosol (LC3-I) to the autophagosomal membrane where it is lipidated (LC3-II)) and p62/SQSTM1 (adaptor protein that serves as a link between LC3 and ubiquitinated substrates), (Klionsky et al., 2016) [1]. Data provided include analyses of protein levels of LC3 and p62 by Western-blotting and endogenous immunofluorescence experiments, but also p62 mRNA levels obtained by quantitative PCR (qPCR). To monitor the turnover of these autophagy markers and, thus, measure the flux of this pathway, cells were under starvation conditions and/or treated with bafilomycin A1 (Baf. A1) to block fusion of autophagosomes with lysosomes.

    Originele taal-2English
    Pagina's (van-tot)641-647
    Aantal pagina's7
    TijdschriftData in brief
    Volume7
    DOI's
    StatusPublished - jun.-2016

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