mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines

Rubén Gómez-Sánchez; Sokhna M S Yakhine-Diop; Mario Rodríguez-Arribas; José M Bravo-San Pedro; Guadalupe Martínez-Chacón; Elisabet Uribe-Carretero; Diana C J Pinheiro de Castro; Elisa Pizarro-Estrella; José M Fuentes; Rosa A González-Polo

doi:10.1016/j.dib.2016.02.085

mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines

Rubén Gómez-Sánchez, Sokhna M S Yakhine-Diop, Mario Rodríguez-Arribas, José M Bravo-San Pedro, Guadalupe Martínez-Chacón, Elisabet Uribe-Carretero, Diana C J Pinheiro de Castro, Elisa Pizarro-Estrella, José M Fuentes, Rosa A González-Polo

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We characterized the dynamics of autophagy in vitro using four different cell systems and analyzing markers widely used in this field, i.e. LC3 (microtubule-associated protein 1 light chain 3; protein recruited from the cytosol (LC3-I) to the autophagosomal membrane where it is lipidated (LC3-II)) and p62/SQSTM1 (adaptor protein that serves as a link between LC3 and ubiquitinated substrates), (Klionsky et al., 2016) [1]. Data provided include analyses of protein levels of LC3 and p62 by Western-blotting and endogenous immunofluorescence experiments, but also p62 mRNA levels obtained by quantitative PCR (qPCR). To monitor the turnover of these autophagy markers and, thus, measure the flux of this pathway, cells were under starvation conditions and/or treated with bafilomycin A1 (Baf. A1) to block fusion of autophagosomes with lysosomes.

Originele taal-2	English
Pagina's (van-tot)	641-647
Aantal pagina's	7
Tijdschrift	Data in brief
Volume	7
DOI's	https://doi.org/10.1016/j.dib.2016.02.085
Status	Published - jun.-2016

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10.1016/j.dib.2016.02.085Licentie: CC BY

mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell linesFinal publisher's version, 2,42 MBLicentie: CC BY

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Gómez-Sánchez, R., Yakhine-Diop, S. M. S., Rodríguez-Arribas, M., Bravo-San Pedro, J. M., Martínez-Chacón, G., Uribe-Carretero, E., Pinheiro de Castro, D. C. J., Pizarro-Estrella, E., Fuentes, J. M., & González-Polo, R. A. (2016). mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines. Data in brief, 7, 641-647. https://doi.org/10.1016/j.dib.2016.02.085

@article{96d0d2fdceb6444f8d2b9eff1e31b72f,

title = "mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines",

abstract = "We characterized the dynamics of autophagy in vitro using four different cell systems and analyzing markers widely used in this field, i.e. LC3 (microtubule-associated protein 1 light chain 3; protein recruited from the cytosol (LC3-I) to the autophagosomal membrane where it is lipidated (LC3-II)) and p62/SQSTM1 (adaptor protein that serves as a link between LC3 and ubiquitinated substrates), (Klionsky et al., 2016) [1]. Data provided include analyses of protein levels of LC3 and p62 by Western-blotting and endogenous immunofluorescence experiments, but also p62 mRNA levels obtained by quantitative PCR (qPCR). To monitor the turnover of these autophagy markers and, thus, measure the flux of this pathway, cells were under starvation conditions and/or treated with bafilomycin A1 (Baf. A1) to block fusion of autophagosomes with lysosomes.",

author = "Rub{\'e}n G{\'o}mez-S{\'a}nchez and Yakhine-Diop, \{Sokhna M S\} and Mario Rodr{\'i}guez-Arribas and \{Bravo-San Pedro\}, \{Jos{\'e} M\} and Guadalupe Mart{\'i}nez-Chac{\'o}n and Elisabet Uribe-Carretero and \{Pinheiro de Castro\}, \{Diana C J\} and Elisa Pizarro-Estrella and Fuentes, \{Jos{\'e} M\} and Gonz{\'a}lez-Polo, \{Rosa A\}",

year = "2016",

month = jun,

doi = "10.1016/j.dib.2016.02.085",

language = "English",

volume = "7",

pages = "641--647",

journal = "Data in brief",

issn = "2352-3409",

publisher = "Elsevier",

}

Gómez-Sánchez, R, Yakhine-Diop, SMS, Rodríguez-Arribas, M, Bravo-San Pedro, JM, Martínez-Chacón, G, Uribe-Carretero, E, Pinheiro de Castro, DCJ, Pizarro-Estrella, E, Fuentes, JM & González-Polo, RA 2016, 'mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines', Data in brief, vol. 7, blz. 641-647. https://doi.org/10.1016/j.dib.2016.02.085

TY - JOUR

T1 - mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines

AU - Gómez-Sánchez, Rubén

AU - Yakhine-Diop, Sokhna M S

AU - Rodríguez-Arribas, Mario

AU - Bravo-San Pedro, José M

AU - Martínez-Chacón, Guadalupe

AU - Uribe-Carretero, Elisabet

AU - Pinheiro de Castro, Diana C J

AU - Pizarro-Estrella, Elisa

AU - Fuentes, José M

AU - González-Polo, Rosa A

PY - 2016/6

Y1 - 2016/6

N2 - We characterized the dynamics of autophagy in vitro using four different cell systems and analyzing markers widely used in this field, i.e. LC3 (microtubule-associated protein 1 light chain 3; protein recruited from the cytosol (LC3-I) to the autophagosomal membrane where it is lipidated (LC3-II)) and p62/SQSTM1 (adaptor protein that serves as a link between LC3 and ubiquitinated substrates), (Klionsky et al., 2016) [1]. Data provided include analyses of protein levels of LC3 and p62 by Western-blotting and endogenous immunofluorescence experiments, but also p62 mRNA levels obtained by quantitative PCR (qPCR). To monitor the turnover of these autophagy markers and, thus, measure the flux of this pathway, cells were under starvation conditions and/or treated with bafilomycin A1 (Baf. A1) to block fusion of autophagosomes with lysosomes.

AB - We characterized the dynamics of autophagy in vitro using four different cell systems and analyzing markers widely used in this field, i.e. LC3 (microtubule-associated protein 1 light chain 3; protein recruited from the cytosol (LC3-I) to the autophagosomal membrane where it is lipidated (LC3-II)) and p62/SQSTM1 (adaptor protein that serves as a link between LC3 and ubiquitinated substrates), (Klionsky et al., 2016) [1]. Data provided include analyses of protein levels of LC3 and p62 by Western-blotting and endogenous immunofluorescence experiments, but also p62 mRNA levels obtained by quantitative PCR (qPCR). To monitor the turnover of these autophagy markers and, thus, measure the flux of this pathway, cells were under starvation conditions and/or treated with bafilomycin A1 (Baf. A1) to block fusion of autophagosomes with lysosomes.

U2 - 10.1016/j.dib.2016.02.085

DO - 10.1016/j.dib.2016.02.085

M3 - Article

C2 - 27054171

SN - 2352-3409

VL - 7

SP - 641

EP - 647

JO - Data in brief

JF - Data in brief

ER -

mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines

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