TY - GEN
T1 - Multi-modal analysis of tumor-derived extracellular vesicles immunocaptured from plasma
AU - Beekman, Pepijn
AU - Enciso-Martinez, Agustin
AU - Piontek, Melissa
AU - Terstappen, Leon
AU - Roos, Wouter
AU - Otto, Cees
AU - Le Gac, Séverine
N1 - Funding Information:
This work has been conducted as part of the Perspectief Program Cancer ID with project numbers 14192, 14193 and 14196, which is funded by the Netherlands Organization for Scientific Research (NWO).
Publisher Copyright:
© 2020 CBMS-0001
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020
Y1 - 2020
N2 - Extracellular vesicles have emerged in recent years as highly promising for understanding cell communication, drug delivery, and medical applications. Specifically, tumor-derived extracellular vesicles (tdEVs) have demonstrated excellent prognostic value in cancer diagnostics compared to imaging approaches. Despite the growing body of expertise regarding EVs, great challenges remain, notably in their handling and characterization. In complex media, other particles with similar characteristics may occlude measurements. Here, a platform is presented for the immunocapturing of tdEVs for identifying their origin followed by further multi-modal analysis by Raman spectroscopy, confocal microscopy and atomic force microscopy (AFM).
AB - Extracellular vesicles have emerged in recent years as highly promising for understanding cell communication, drug delivery, and medical applications. Specifically, tumor-derived extracellular vesicles (tdEVs) have demonstrated excellent prognostic value in cancer diagnostics compared to imaging approaches. Despite the growing body of expertise regarding EVs, great challenges remain, notably in their handling and characterization. In complex media, other particles with similar characteristics may occlude measurements. Here, a platform is presented for the immunocapturing of tdEVs for identifying their origin followed by further multi-modal analysis by Raman spectroscopy, confocal microscopy and atomic force microscopy (AFM).
KW - Cancer diagnostics
KW - Extracellular vesicles
KW - Multi-modal analysis
KW - Surface modification
KW - TdEVs
UR - http://www.scopus.com/inward/record.url?scp=85098291464&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85098291464
T3 - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 404
EP - 405
BT - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
Y2 - 4 October 2020 through 9 October 2020
ER -