Novel RANKL DE-loop mutants antagonize RANK-mediated osteoclastogenesis

Yizhou Wang, Aart H G van Assen, Carlos Ricardo Rodrigues Dos Reis, Rita Setroikromo, Ronald van Merkerk, Ykelien L Boersma, Robbert H. Cool, Wim J Quax

OnderzoeksoutputAcademicpeer review

6 Citaten (Scopus)
264 Downloads (Pure)

Samenvatting

Bone is a dynamic tissue that is maintained by continuous renewal. An imbalance in bone resorption and bone formation can lead to a range of disorders, such as osteoporosis. The receptor activator of NF-kappa B (RANK)-RANK-ligand (RANKL) pathway plays a major role in bone remodeling. Here, we investigated the effect of mutations at position I248 in the DE-loop of murine RANKL on the interaction of RANKL with RANK, and subsequent activation of osteoclastogenesis. Two single mutants, RANKL I248Y and I248K, were found to maintain binding and have the ability to reduce wild-type RANKL-induced osteoclastogenesis. The generation of RANK-antagonists is a promising strategy for the exploration of new therapeutics against osteoporosis.

Originele taal-2English
Pagina's (van-tot)2501-2512
Aantal pagina's12
TijdschriftFebs Journal
Volume284
Nummer van het tijdschrift15
DOI's
StatusPublished - aug-2017

Citeer dit