Novel Zinc-Related Differentially Methylated Regions in Leukocytes of Women With and Without Obesity

Natalia Yumi Noronha, Mariana Barato, Chanachai Sae-Lee, Marcela Augusta de Souza Pinhel, Ligia Moriguchi Watanabe, Vanessa Aparecida Batista Pereira, Guilherme da Silva Rodrigues, Deborah Araujo Morais, Wellington Tavares de Sousa, Vanessa Cristina de Oliveira Souza, Jessica Rodrigues Placa, Wilson Salgado Jr, Fernando Barbosa, Torsten Plosch, Carla Barbosa Nonino*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

2 Citaten (Scopus)
6 Downloads (Pure)


IntroductionNutriepigenetic markers are predictive responses associated with changes in "surrounding" environmental conditions of humans, which may influence metabolic diseases. Although rich in calories, Western diets could be linked with the deficiency of micronutrients, resulting in the downstream of epigenetic and metabolic effects and consequently in obesity. Zinc (Zn) is an essential nutrient associated with distinct biological roles in human health. Despite the importance of Zn in metabolic processes, little is known about the relationship between Zn and epigenetic. Thus, the present study aimed to identify the epigenetic variables associated with Zn daily ingestion (ZnDI) and serum Zinc (ZnS) levels in women with and without obesity. Materials and MethodsThis is a case-control, non-randomized, single-center study conducted with 21 women allocated into two groups: control group (CG), composed of 11 women without obesity, and study group (SG), composed of 10 women with obesity. Anthropometric measurements, ZnDI, and ZnS levels were evaluated. Also, leukocyte DNA was extracted for DNA methylation analysis using 450 k Illumina BeadChips. The epigenetic clock was calculated by Horvath method. The chip analysis methylation pipeline (ChAMP) package selected the differentially methylated regions (DMRs). ResultsThe SG had lower ZnS levels than the CG. Moreover, in SG, the ZnS levels were negatively associated with the epigenetic age acceleration. The DMR analysis revealed 37 DMRs associated with ZnDI and ZnS levels. The DMR of PM20D1 gene was commonly associated with ZnDI and ZnS levels and was hypomethylated in the SG. ConclusionOur findings provide new information on Zn's modulation of DNA methylation patterns and bring new perspectives for understanding the nutriepigenetic mechanisms in obesity.

Originele taal-2English
Aantal pagina's10
TijdschriftFrontiers in nutrition
StatusPublished - 7-mrt-2022

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