Nuclear matrix as a target for hyperthermic killing of cancer cells

JLR Roti*, HH Kampinga, RS Malyapa, WD Wright, RP vanderWaal

*Bijbehorende auteur voor dit werk

    OnderzoeksoutputAcademicpeer review

    100 Citaten (Scopus)

    Samenvatting

    The nuclear matrix organizes nuclear DNA into operational domains in which DNA is undergoing replication, transcription or is inactive. The proteins of the nuclear matrix are among the most thermal labile proteins in the cell, undergoing denaturation at temperatures as low as 43-45 degrees C, i.e. relevant temperatures for the clinical treatment of cancer. Heat shock-induced protein denaturation results in the aggregation of proteins to the nuclear matrix. Protein aggregation with the nuclear matrix is associated with the disruption of many nuclear matrix-dependent functions (e.g. DNA replication, DNA transcription, hnRNA processing, DNA repair, etc.) and cell death. Heat shock proteins are believed to bind denatured proteins and either prevents aggregation or render aggregates more readily dissociable. While many studies suggest a role for Hsp70 in heat resistance, we have recently found that nuclear localization/delocalization of Hsp70 and its rate of synthesis, but not its amount, correlate with a tu mor cell's ability to proliferate at 41.1 degrees C. These results imply that not only is the nuclear matrix a target for the lethal effects of heat, but it also is a target for the protective, chaperoning and/or enhanced recovery effects of heat shock proteins.

    Originele taal-2English
    Pagina's (van-tot)245-255
    Aantal pagina's11
    TijdschriftCell stress & chaperones
    Volume3
    Nummer van het tijdschrift4
    StatusPublished - dec-1998

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