On the Dissimilarity of 5′-AMP Induced Hypothermia and Torpor in Mice

A.M. Strijkstra, T. Koopmans, H.R. Bouma, S.F. de Boer, R.A. Hut, A.S. Boerema

OnderzoeksoutputAcademicpeer review

Samenvatting

Administration of adenosine-5′-monophosphate (5′-AMP) can induce an artificial but endogenously reversible torpor-like state in mice. The dynamics of body temperature and the relation between body temperature and metabolic rate may indicate the (dis)similarity of this artificial torpor-like state to natural torpor in intact animals. We investigated these in C57BL/6J mice by (1) comparing cooling rates during 5′-AMP induced hypothermia to cooling rates during high workload induced torpor, and by (2) estimating the relative contributions of metabolic suppression and passive temperature (Q 10) effects in the 5′-AMP induced hypothermic state. We did the latter by back-extrapolating the relation between body temperature and metabolic rate in hypothermic conditions to the euthermic temperature level, using calculated Q 10-values. The data indicate that (1) cooling rate in 5′-AMP induced hypothermia is about 1.8 times faster than in natural torpor in workload conditions, and that (2) Q 10 effects can entirely explain the metabolic reduction of 5′-AMP induced hypothermia, indicating that active metabolic suppression may be lacking. Together, this suggests fundamental differences between 5′-AMP induced hypothermia and natural torpor, limiting the validity of the paradigm to the study of effects of hypothermic conditions and temperature related metabolic effects.
Originele taal-2English
TitelLiving in a Seasonal World
SubtitelThermoregulatory and Metabolic Adaptations
RedacteurenW. Arnold, C. Bieber, E. Millesi, T. Ruf
Plaats van productieBerlin, Heidelberg
UitgeverijSpringer Verlag
Hoofdstuk31
Pagina's351-362
Aantal pagina's12
ISBN van elektronische versie9783642286780
ISBN van geprinte versie9783642286773
DOI's
StatusPublished - 2012

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