Overexpression of Cystathionine gamma-Lyase Suppresses Detrimental Effects of Spinocerebellar Ataxia Type 3

Pauline M. Snijder, Madina Baratashvili, Nicola A. Grzeschik, Henri G. D. Leuvenink, Lucas Kuijpers, Sippie Huitema, Onno Schaap, Ben N. G. Giepmans, Jeroen Kuipers, Jan Lj Miljkovic, Aleksandra Mitrovic, Eelke M. Bos, Csaba Szabo, Harm H. Kampinga, Pascale F. Dijkers, Wilfred F.A. den Dunnen, Milos R. Filipovic, Harry van Goor, Ody C. M. Sibon*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

22 Citaten (Scopus)
225 Downloads (Pure)

Samenvatting

Spinocerebellar ataxia type 3 (SCA3) is a polyglutamine (polyQ) disorder caused by a CAG repeat expansion in the ataxin-3 (ATXN3) gene resulting in toxic protein aggregation. Inflammation and oxidative stress are considered secondary factors contributing to the progression of this neurodegenerative disease. There is no cure that halts or reverses the progressive neurodegeneration of SCA3. Here we show that overexpression of cystathionine.-lyase, a central enzyme in cysteine metabolism, is protective in a Drosophila model for SCA3. SCA3 flies show eye degeneration, increased oxidative stress, insoluble protein aggregates, reduced levels of protein persulfidation and increased activation of the innate immune response. Overexpression of Drosophila cystathionine.-lyase restores protein persulfidation, decreases oxidative stress, dampens the immune response and improves SCA3-associated tissue degeneration. Levels of insoluble protein aggregates are not altered; therefore, the data implicate a modifying role of cystathionine.-lyase in ameliorating the downstream consequence of protein aggregation leading to protection against SCA3-induced tissue degeneration. The cystathionine.-lyase expression is decreased in affected brain tissue of SCA3 patients, suggesting that enhancers of cystathionine.-lyase expression or activity are attractive candidates for future therapies.

Originele taal-2English
Pagina's (van-tot)758-768
Aantal pagina's11
TijdschriftMolecular medicine
Volume21
DOI's
StatusPublished - 2015

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