Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins

Ena Sokol; Miranda Nijenhuis; Klaas A Sjollema; Marcel F Jonkman; Hendri H Pas; Ben N G Giepmans

doi:10.1007/978-1-4939-6786-5_9

Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins

Ena Sokol, Miranda Nijenhuis, Klaas A Sjollema, Marcel F Jonkman, Hendri H Pas, Ben N G Giepmans

Onderzoeksoutput › Academic

2 Citaten (Scopus)

Samenvatting

Particle bombardment of gold microparticles coated with plasmids, which are accelerated to high velocity, is used for transfection of cells within tissue. Using this method, cDNA encoding proteins of interest introduced into ex vivo living human skin enables studying of proteins of interest in real time. Here, technical aspects of particle bombardment of ex vivo skin are described using green fluorescent protein (GFP) as readout for efficiency. This method can be applied on numerous tissues, including in living model animals.

Originele taal-2	English
Titel	Inflammation
Subtitel	Methods and Protocols
Redacteuren	Björn E. Clausen, Jon D. Laman
Uitgeverij	Springer
Pagina's	107-118
Aantal pagina's	12
Volume	1559
Uitgave	1
ISBN van elektronische versie	978-1-4939-6786-5
ISBN van geprinte versie	978-1-4939-6784-1
DOI's	https://doi.org/10.1007/978-1-4939-6786-5_9
Status	Published - 2017

Toegang tot document

10.1007/978-1-4939-6786-5_9

Handle.net

Permanente koppeling

Citeer dit

@inbook{333a4e3cd5d24cd4acd9d91444738c6f,

title = "Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins",

abstract = "Particle bombardment of gold microparticles coated with plasmids, which are accelerated to high velocity, is used for transfection of cells within tissue. Using this method, cDNA encoding proteins of interest introduced into ex vivo living human skin enables studying of proteins of interest in real time. Here, technical aspects of particle bombardment of ex vivo skin are described using green fluorescent protein (GFP) as readout for efficiency. This method can be applied on numerous tissues, including in living model animals.",

author = "Ena Sokol and Miranda Nijenhuis and Sjollema, {Klaas A} and Jonkman, {Marcel F} and Pas, {Hendri H} and Giepmans, {Ben N G}",

year = "2017",

doi = "10.1007/978-1-4939-6786-5_9",

language = "English",

isbn = "978-1-4939-6784-1",

volume = "1559",

pages = "107--118",

editor = "Clausen, {Bj{\"o}rn E.} and Laman, {Jon D.}",

booktitle = "Inflammation",

publisher = "Springer",

edition = "1",

}

TY - CHAP

T1 - Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins

AU - Sokol, Ena

AU - Nijenhuis, Miranda

AU - Sjollema, Klaas A

AU - Jonkman, Marcel F

AU - Pas, Hendri H

AU - Giepmans, Ben N G

PY - 2017

Y1 - 2017

N2 - Particle bombardment of gold microparticles coated with plasmids, which are accelerated to high velocity, is used for transfection of cells within tissue. Using this method, cDNA encoding proteins of interest introduced into ex vivo living human skin enables studying of proteins of interest in real time. Here, technical aspects of particle bombardment of ex vivo skin are described using green fluorescent protein (GFP) as readout for efficiency. This method can be applied on numerous tissues, including in living model animals.

AB - Particle bombardment of gold microparticles coated with plasmids, which are accelerated to high velocity, is used for transfection of cells within tissue. Using this method, cDNA encoding proteins of interest introduced into ex vivo living human skin enables studying of proteins of interest in real time. Here, technical aspects of particle bombardment of ex vivo skin are described using green fluorescent protein (GFP) as readout for efficiency. This method can be applied on numerous tissues, including in living model animals.

U2 - 10.1007/978-1-4939-6786-5_9

DO - 10.1007/978-1-4939-6786-5_9

M3 - Chapter

C2 - 28063041

SN - 978-1-4939-6784-1

VL - 1559

SP - 107

EP - 118

BT - Inflammation

A2 - Clausen, Björn E.

A2 - Laman, Jon D.

PB - Springer

ER -