Peptiligase, an Enzyme for Efficient Chemoenzymatic Peptide Synthesis and Cyclization in Water

Ana Toplak, Timo Nuijens, Peter J. L. M. Quaedflieg, Bian Wu, Dick B. Janssen

OnderzoeksoutputAcademicpeer review

46 Citaten (Scopus)
39 Downloads (Pure)


We describe a novel, organic cosolvent-stable and cation-independent engineered enzyme for peptide coupling reactions. The enzyme is a variant of a stable calcium-independent mutant of subtilisin BPN, with the catalytic Ser212 mutated to Cys and Pro216 converted to Ala. The enzyme, called peptiligase, catalyzes exceptionally efficient peptide coupling in water with a surprisingly high synthesis over hydrolysis (S/H) ratio. The S/H ratio of the peptide ligation reaction is correlated to the length of the peptide substrate and proved to be >100 for the synthesis of a 13-mer peptide, which corresponds to >99% conversion to the ligated peptide product and

Originele taal-2English
Pagina's (van-tot)2140-2147
Aantal pagina's8
TijdschriftAdvanced Synthesis & Catalysis
Nummer van het tijdschrift13
StatusPublished - 30-jun.-2016

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