Perfusion Decellularization of Extrahepatic Bile Duct Allows Tissue-Engineered Scaffold Generation by Preserving Matrix Architecture and Cytocompatibility

Yolik Ramírez-Marín, David Eduardo Abad-Contreras, Martha Ustarroz-Cano, Norma S. Pérez-Gallardo, Lorena Villafuerte-García, Dulce Maria Puente-Guzmán, Jorge Luna del Villar-Velasco, Leonardo Alejandro Rodríguez-López, Gonzalo Torres-Villalobos, Miguel Ángel Mercado, Jesús Tapia-Jurado, Francisco Drusso Martínez-García, Martin Conrad Harmsen, M. Cristina Piña-Barba, David M. Giraldo-Gomez*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

1 Citaat (Scopus)
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Samenvatting

Reconstruction of bile ducts damaged remains a vexing medical problem. Surgeons have few options when it comes to a long segment reconstruction of the bile duct. Biological scaffolds of decellularized biliary origin may offer an approach to support the replace of bile ducts. Our objective was to obtain an extracellular matrix scaffold derived from porcine extrahepatic bile ducts (dECM-BD) and to analyze its biological and biochemical properties. The efficiency of the tailored perfusion decellularization process was assessed through histology stainings. Results from 4’-6-diamidino-2-phenylindole (DAPI), Hematoxylin and Eosin (H&E) stainings, and deoxyribonucleic acid (DNA) quantification showed proper extracellular matrix (ECM) decellularization with an effectiveness of 98%. Immunohistochemistry results indicate an effective decrease in immunogenic marker as human leukocyte antigens (HLA-A) and Cytokeratin 7 (CK7) proteins. The ECM of the bile duct was preserved according to Masson and Herovici stainings. Data derived from scanning electron microscopy (SEM) and thermogravimetric analysis (TGA) showed the preservation of the dECM-BD hierarchical structures. Cytotoxicity of dECM-BD was null, with cells able to infiltrate the scaffold. In this work, we standardized a decellularization method that allows one to obtain a natural bile duct scaffold with hierarchical ultrastructure preservation and adequate cytocompatibility.
Originele taal-2English
Artikelnummer3099
Aantal pagina's15
TijdschriftMaterials
Volume14
Nummer van het tijdschrift11
DOI's
StatusPublished - 5-jun-2021

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