Peroxisome development in yeast is associated with the formation of Pex3-dependent peroxisome-vacuole contact sites

Huala Wu, Rinse de Boer, Arjen M Krikken, Arman Akşit, Wei Yuan, Ida J van der Klei*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

8 Citaten (Scopus)
52 Downloads (Pure)


Using electron and fluorescence microscopy techniques, we identified various physical contacts between peroxisomes and other cell organelles in the yeast Hansenula polymorpha. In exponential glucose-grown cells, which typically contain a single small peroxisome, contacts were only observed with the endoplasmic reticulum and the plasma membrane. Here we focus on a novel peroxisome-vacuole contact site that is formed when glucose-grown cells are shifted to methanol containing media, conditions that induce strong peroxisome development. At these conditions, the small peroxisomes rapidly increase in size, a phenomenon that is paralleled by the formation of distinct intimate contacts with the vacuole. Localization studies showed that the peroxin Pex3 accumulated in patches at the peroxisome-vacuole contact sites. In wild-type cells growing exponentially on medium containing glucose, peroxisome-vacuole contact sites were never observed. However, upon overproduction of Pex3 peroxisomes also associated to vacuoles at these growth conditions. Our observations strongly suggest a role for Pex3 in the formation of a novel peroxisome-vacuole contact site. This contact likely plays a role in membrane growth as it is formed solely at conditions of strong peroxisome expansion.

Originele taal-2English
Pagina's (van-tot)349-359
Aantal pagina's11
TijdschriftBiochimica et Biophysica Acta (BBA) - Molecular Cell Research
Nummer van het tijdschrift3
StatusPublished - mrt-2019

Citeer dit