Pex19p Contributes to Peroxisome Inheritance in the Association of Peroxisomes to Myo2p

Marleen Otzen, Robert Rucktaeschel, Sven Thoms, Kerstin Ernmrich, Arjen M. Krikken, Ralf Erdmann, Ida J. van der Klei*, Kerstin Emmrich, A.M. Krikken

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

20 Citaten (Scopus)
712 Downloads (Pure)

Samenvatting

During budding of yeast cells peroxisomes are distributed over mother cell and bud, a process that involves the myosin motor protein Myo2p and the peroxisomal membrane protein Inp2p. Here, we show that Pex19p, a peroxin implicated in targeting and complex formation of peroxisomal membrane proteins, also plays a role in peroxisome partitioning. Binding studies revealed that Pex19p interacts with the cargo-binding domain of Myo2p. We identified mutations in Myo2p that specifically reduced binding to Pex19p, but not to Inp2p. The interaction between Myo2p and Pex19p was also reduced by a mutation that blocked Pex19p farnesylation. Microscopy revealed that the Pex19p-Myo2p interaction is important for peroxisome inheritance, because mutations that affect this interaction hamper peroxisome inheritance in vivo. Together these data suggest that both Inp2p and Pex19p are required for proper association of peroxisomes to Myo2p.

Originele taal-2English
Pagina's (van-tot)947-959
Aantal pagina's13
TijdschriftTraffic
Volume13
Nummer van het tijdschrift7
DOI's
StatusPublished - jul.-2012

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