Samenvatting
Fluorescent nanodiamonds are a useful for biosensing of intracellular signaling networks or environmental changes (such as temperature, pH or free radical generation). HeLa cells are interesting to study with these nanodiamonds since they are a model cell system that is widely used to study cancer-related diseases. However, they only internalize low numbers of nanodiamond particles very slowly via the endocytosis pathway. In this work, we show that pH-sensitive, dextran-coated fluorescent nanodiamonds can be used to visualise this pathway. Additionally, this coating improved diamond uptake in HeLa cells by 5.3 times (*** p < 0.0001) and decreased the required time for uptake to only 30 min. We demonstrated further that nanodiamonds enter HeLa cells via endolysosomes and are eventually expelled by cells.
Originele taal-2 | English |
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Artikelnummer | 1837 |
Pagina's (van-tot) | 1-11 |
Aantal pagina's | 11 |
Tijdschrift | Nanomaterials |
Volume | 11 |
Nummer van het tijdschrift | 7 |
DOI's | |
Status | Published - 15-jul.-2021 |