Photocontrolled Fluorescence "Double-Check" Bioimaging Enabled by a Glycoprobe-Protein Hybrid

Youxin Fu, Hai-Hao Han, Junji Zhang*, Xiao-Peng He*, Ben L. Feringa, He Tian*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

98 Citaten (Scopus)
55 Downloads (Pure)


Despite the rapid development of imaging techniques, precise probe localization and modulation in living cells is still a challenging task. Here we show that the simple hybridization between a photochromic fluorescent glycoprobe and human serum albumin (HSA) enables a unique fluorescence "double-check" mechanism for precisely localizing and manipulating probe molecules in living cells. Docking of a carbohydrate-modified naphthalimide (Naph)-spiropyran (SP) dyad to a hydrophobic pocket of HSA produces the glycoprobe-protein hybrid, causing the protein conformation to fold as determined by small-angle X-ray scattering. We show that the Naph and merocyanine (the photo isomer of SP) fluorescence of the resulting hybrid can be reversibly switched by light in buffer solution and in target cells overexpressing the carbohydrate receptor.

Originele taal-2English
Pagina's (van-tot)8671-8674
Aantal pagina's4
TijdschriftJournal of the American Chemical Society
Nummer van het tijdschrift28
StatusPublished - 18-jul.-2018

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