Polyphenol oxidase-based electrochemical biosensors: A review

Paulo A. Raymundo-Pereira, Tiago A. Silva, Fábio R. Caetano, Laís Ribovski, Eduardo Zapp, Daniela Brondani, Marcio F. Bergamini, Luiz H. Marcolino, Craig E. Banks, Osvaldo N. Oliveira, Bruno C. Janegitz*, Orlando Fatibello-Filho*

*Bijbehorende auteur voor dit werk

Onderzoeksoutputpeer review

44 Citaten (Scopus)

Samenvatting

The detection of phenolic compounds is relevant not only for their possible benefits to human health but also for their role as chemical pollutants, including as endocrine disruptors. The required monitoring of such compounds on-site or in field analysis can be performed with electrochemical biosensors made with polyphenol oxidases (PPO). In this review, we describe biosensors containing the oxidases tyrosinase and laccase, in addition to crude extracts and tissues from plants as enzyme sources. From the survey in the literature, we found that significant advances to obtain sensitive, robust biosensors arise from the synergy reached with a diversity of nanomaterials employed in the matrix. These nanomaterials are mostly metallic nanoparticles and carbon nanostructures, which offer a suitable environment to preserve the activity of the enzymes and enhance electron transport. Besides presenting a summary of contributions to electrochemical biosensors containing PPOs in the last five years, we discuss the trends and challenges to take these biosensors to the market, especially for biomedical applications.

Originele taal-2English
Pagina's (van-tot)198-221
Aantal pagina's24
TijdschriftAnalytica Chimica Acta
Volume1139
DOI's
StatusPublished - 1-dec.-2020
Extern gepubliceerdJa

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