Positioning Bacillus subtilis as terpenoid cell factory

Hegar Pramastya, Yafeng Song, Elfahmi Yaman, Sukrasno Sukrasno, Wim J Quax*

*Bijbehorende auteur voor dit werk

Onderzoeksoutputpeer review

15 Citaten (Scopus)
158 Downloads (Pure)


Increasing demands for bioactive compounds have motivated researchers to employ micro-organisms to produce complex natural products. Currently, Bacillus subtilis has been attracting lots of attention to be developed into terpenoids cell factories due to its generally recognized safe status and high isoprene precursor biosynthesis capacity by endogenous methylerythritol phosphate (MEP) pathway. In this review, we describe the up-to-date knowledge of each enzyme in MEP pathway and the subsequent steps of isomerization and condensation of C5 isoprene precursors. In addition, several representative terpene synthases expressed in B. subtilis and the engineering steps to improve corresponding terpenoids production are systematically discussed. Furthermore, the current available genetic tools are mentioned as along with promising strategies to improve terpenoids in B. subtilis, hoping to inspire future directions in metabolic engineering of B. subtilis for further terpenoid cell factory development.

Originele taal-2English
Pagina's (van-tot)1839-1856
Aantal pagina's18
TijdschriftJournal of Applied Microbiology
Nummer van het tijdschrift6
Vroegere onlinedatum24-okt.-2020
StatusPublished - jun.-2021

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