Prediction of non-alcoholic fatty-liver disease and liver fat content by serum molecular lipids

Matej Oresic*, Tuulia Hyotylainen, Anna Kotronen, Peddinti Gopalacharyulu, Heli Nygren, Johanna Arola, Sandra Castillo, Ismo Mattila, Antti Hakkarainen, Ronald J. H. Borra, Miikka-Juhani Honka, An Verrijken, Sven Francque, Patricia Iozzo, Marja Leivonen, Nabil Jaser, Anne Juuti, Thorkild I. A. Sorensen, Pirjo Nuutila, Luc Van GaalHannele Yki-Jarvinen

*Corresponding author voor dit werk

    OnderzoeksoutputAcademicpeer review

    138 Citaten (Scopus)
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    Samenvatting

    We examined whether analysis of lipids by ultra-performance liquid chromatography (UPLC) coupled to MS allows the development of a laboratory test for non-alcoholic fatty-liver disease (NAFLD), and how a lipid-profile biomarker compares with the prediction of NAFLD and liver-fat content based on routinely available clinical and laboratory data.

    We analysed the concentrations of molecular lipids by UPLC-MS in blood samples of 679 well-characterised individuals in whom liver-fat content was measured using proton magnetic resonance spectroscopy (H-1-MRS) or liver biopsy. The participants were divided into biomarker-discovery (n = 287) and validation (n = 392) groups to build and validate the diagnostic models, respectively.

    Individuals with NAFLD had increased triacylglycerols with low carbon number and double-bond content while lysophosphatidylcholines and ether phospholipids were diminished in those with NAFLD. A serum-lipid signature comprising three molecular lipids ('lipid triplet') was developed to estimate the percentage of liver fat. It had a sensitivity of 69.1% and specificity of 73.8% when applied for diagnosis of NAFLD in the validation series. The usefulness of the lipid triplet was demonstrated in a weight-loss intervention study.

    The liver-fat-biomarker signature based on molecular lipids may provide a non-invasive tool to diagnose NAFLD, in addition to highlighting lipid molecular pathways involved in the disease.

    Originele taal-2English
    Pagina's (van-tot)2266-2274
    Aantal pagina's9
    TijdschriftDiabetologia
    Volume56
    Nummer van het tijdschrift10
    DOI's
    StatusPublished - okt.-2013

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